With 9 figures and 4 tables Summary This study’s aim was to determine the optimal scan parameters for imaging the middle and inner ear of the cat with micro‐computertomography (μCT). Besides, the study set out to assess whether adequate image quality can be obtained to use μCT in diagnostics and research on cat ears. For optimisation, μCT imaging of two cat skull preparations was performed using 36 different scanning protocols. The μCT‐scans were evaluated by four experienced experts with regard to the image quality and detail detectability. By compiling a ranking of the results, the best possible scan parameters could be determined. From a third cat’s skull, a μCT‐scan, using these optimised scan parameters, and a comparative clinical CT‐scan were acquired. Afterwards, histological specimens of the ears were produced which were compared to the μCT‐images. The comparison shows that the osseous structures are depicted in detail. Although soft tissues cannot be differentiated, the osseous structures serve as valuable spatial orientation of relevant nerves and muscles. Clinical CT can depict many anatomical structures which can also be seen on μCT‐images, but these appear a lot less sharp and also less detailed than with μCT.