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Surfactants containing potential donor ligands can coordinate with metal ions and such compounds can beused for the removal of toxic metals and organometallic compounds from aqueous systems. Micelles haveplayed role as soil and water decontamination agents. Micellar solutions and microemulsions are efficientenvironmentally-friendly agents for cleaning artworks, especially for the selective removal of aged polymericcoatings from the surface of wall paintings. Thus micelles have great importance in chemistry. Presentwork explains the synthesis of ligand (4-(Diethylamino) benzaldehyde thiosemicarbazone) by conventionalas well as green method, volumetric analysis and antimicrobial investigations on Cu (II)- Thiosemicarbazone complexes. Solution studies on the complexes have also been carried out in different micellar (SDS, HTAB,TX-100 ) systems at 25 °C. Stability constants have been determined in 60% ethanol by pH metric study. Protonligand stability constant and metal-ligand stability have been determined potentiometrically. Antibacterialinvestigations have been carried out against, S. aureus, B.substilis bacterial strain.