Growth promoting Rhizobium spp are frequently used as biofertilizers for agricultural cropping system. Furthermore, Isolation, screening and biochemical characterization of Rhizobium for a specific plant is necessary to examine ability of isolated bacteria to affect the growth and development of host plant in various ways. The current study was aimed to isolate plant specific rhizobacterial strains which are compatible with lentil (Lens culinaris Medik.L.) plant. 20 bacterial isolates have been isolated from root nodules of lentil from various agro ecological area and their biochemical characterization was performed by different plant growth promotion activities. The result showed that, among 20 isolates, four isolates have vigorous plant growth promoting activities. Four bacterial strains were able to solubilise phosphorous along with hormone production. Moreover, among four bacterial strains, two strongly produced HCN and siderophore in vitro. Subsequently, all selected bacterial isolates were inoculated in lentil seeds of variety HUL57 to study germination percentage and vigour index of the crop. Out of four isolates 26N isolate performed best growth promotion activities on lentil seedlings. Finally, on the basis of performance of bacteria on plant, four isolates were characterized using molecular approach of species identification such as 16S rRNA sequencing.