Abstract. Zahra U, Kartika Y, Batubara I, Darusman LK, Maddu A. 2016. Short Communication: Screening the potency of . Zingiberaceae is a family of plant with antioxidant, anticancer, and antiaging activities. The use of the leaves part of this family has never been explored. Therefore, the aim of this research is to evaluate the antioxidant and antiaging activities of some Zingiberaceae's leaves. The species of Zingiberaceae used in this study are Alpinia galanga (L), Curcuma aeruginosa Roxb, Curcuma longa L, Curcuma zedoaria, Curcuma xanthorrhiza Roxb, Boesenbergia rotunda (L.), Elettaria cardamomum, Zingiber zerumbet, Zingiber officinale Roscoe, and Zingiber purpureum Roscoe. Leaves were extracted with 2 different solvents sequentially: ethyl acetate and methanol. Total phenolic and flavonoid contents of the extracts were determined spectrophotometrically. Antioxidant activities were tested by using DPPH (2,2-diphenyl-1-picrylhydrazyl ) and ABTS (2,2'-azino-bis-3-ethylbenzothiazolin-6-sulfonic acid), whilst antiaging activity was tested by using antiglycation method. The results showed that the flavonoid content of the extracts were in the range of 0.83-25.17 g QE/100 g extract with total phenolic content ranging from 9.80-91.71 GAE mg/100 mg extract. The methanol extract of Curcuma xanthorrhiza displayed the highest antioxidant capacity in the DDPH (IC 50 282.35 mg/mL) and TEAC (10.08%) assays, followed by the ethyl acetate extract of C. longa, ethyl acetic extract of C. xanthorrhiza and methanol extract of Z. officinale, while the most active antiglycation is the methanol extract of Z. officinale (IC 50 203.85 mg/L).

Rimpang keluarga Zingiberaceae dilaporkan memiliki aroma khas dan juga aktif sebagai antioksidan, sementara daun keluarga ini memiliki aroma khas yang mirip dengan rimpangnya. Oleh karena itu, penelitian ini bertujuan untuk mengisolasi minyak atsiri daun beberapa spesies dari keluarga Zingiberaceae dan menentukan aktivitas minyak atsiri tersebut sebagai antioksidan dan antiglikasi yang berhubungan dengan anti-penuaan. Daun dari 8 spesies yaitu Alpinia galanga, Boesenbergia pandaratum, Curcuma aeruginosa, Curcuma domestica, Curcuma xanthorrhiza, Curcuma zedoaria, Ellettaria cardamomum, dan Zingiber officinale diisolasi minyak atsirinya menggunakan teknik distilasi uap. Minyak yang diperoleh ditentukan kemampuannya sebagai antioksidan menggunakan metode ABTS (2,2'-azino-bis-3ethylbenzothiazolin-6-sulfonic acid) menggunakan spektrofotometer visual serta kemampuan antiaging melalui aktivitas antiglikasi mengunakan flourimetri. Rendemen minyak dihasilkan mulai dari 0.04 hingga 3.15%. Kapasitas antioksidan tertinggi dengan metode ABTS ditemukan pada minyak atsiri daun Curcuma aeruginosa sebesar 5.10g ekuivalen asam askorbat/ 100 g minyak sedangkan minyak dengan aktivitas antiglikasi terbesar ditemukan pada minyak daun Z. officinale dengan konsentrasi penghambatan 50%, IC50 sebesar 207.95mg/L. Senyawa kimia pada minyak atsiri daun Z. officinale ditentukan menggunakan metode Kromatografi Gas-Spektrometri Massa dan ditemukan kariofilena sebagai komponen dominannya. Kariofilena mampu bertindak sebagai antiglikasi dengan konsentrasi penghambatan 50% sebesar 113.8 µM. Minyak atsiri dari daun Z officinale berpotensi dikembangkan sebagai antiaging.

MFC is a bio-electrochemical system driving an electric current by using high-energy bacteria and oxidants. This research aimed to investigate the effect of electrolyte and buffer on electrical production using Pseudomonas sp. In Molasses substrate. The method in this research was the double compartment that consist of anode and cathode chambers. Both were related by salt bridge. This study showed that the addition of a combination of KMnO4 electrolyte solution with sodium phosphate buffer solution obtained a maximum potential difference of 0.67 V. The result also revealed that the combination of KMnO4 electrolyte solution with Potassium Phosphate Buffer solution produced a 1.44 mA maximum current with power density of 660.82 mW/m2.

Microbial Fuel Cell (MFC) was a Technology that produced electrical energy utilizing microorganism. The purpose of this study was to know enceng gondok potency as substrate in MFC system and add electrolyte solution and buffer. This study used enceng gondok as substrate and Pseudomonas sp. bacteria. The Method of this study was using double compartment that consist of anode and cathode chambers. Both of them were related by salt bridge. The result showed that electric current and potential differences maximum that was produced (without added of electrolyte solution and buffer) was 0.11 mA and 0,27 V while power density was 20.34 mW/m2. Whereas Electrolyte solution and buffer combination showed electric current and potential differences maximum that produced was 3.06 mA and 0.86 V while power density yaitu 1802.46 mW/m2.

Jarak merah merupakaan salah satu tanaman endemik Indonesia. Kandungan metabolit sekunder yang ada pada tanaman ini berpotensi untuk dijadikan sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui potensi metabolit sekunder ekstrak jarak merah dalam menghambat pertumbuhan bakteri Propinium acne. Metode pengujian meliputi skrining fitokimia dan pengujian antibakteri dilakukan dengan metode sumuran. Hasil penelitian menunjukkan bahwa ekstrak daun jarak merah memiliki metabolit sekunder yaitu flavonoid, alkaloid dan steroid. Ekstrak etil asetat dan etanol daun jarak merah mampu menghambat pertumbuhan bakteri Propionibacterium acne dengan zona hambat berturut-turut sebesar 14,43 mm dan 9,37 mm.