Abstract. Hidayaturrahmah, Mabrur, Santoso HB, Sasmita R, Rahmy USA, Badruzsaufari. 2019. Short Communication: Protein profiles of Giant Mudskipper and its potential use as biomarker candidate for heavy metal contamination in Barito Estuary, Indonesia. Biodiversitas 20: 745-753. Giant mudskipper (Periphthalmodon schlosseri, Pallas 1770) is one of important biological diversity that potential as bio-indicator in environment assessment which is heavy metal contamination. These fish are susceptible to ambient environment, that effect to their physiological mechanism including protein synthesis and expression. This research aims to identify protein profiles of giant mudskipper muscle and review its potential application as a biomarker for heavy metal contamination in Barito Estuary of South Kalimantan. Total proteins were isolated from fish muscle using Tris EDTA buffer and then precipitated using Ammonium sulfate salt. The protein concentration was measured using Bradford assay and then separated based on molecular weight using the SDS-PAGE method. The result showed muscle proteins of giant mudskipper could be precipitated on optimum condition at Ammonium sulfate saturation 60-70% which protein concentration 5.106 mg.mL-1. The protein separated into 20 bands for raw and precipitated proteins which molecular weight ranging from 33 into 184 kDa. Further study is needed for protein identification based on amino acids sequence of protein bands for biomarker discovery and validation.

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Abstract. Sasmita R, Mabrur, Rahmy USA, Badruzsaufari. 2019. Genetic diversity and phylogenetic relationship among Anabantoidei fish (Anabantiformes) in South Kalimantan, Indonesia based on SDS-PAGE analysis. Biodiversitas 20: 2519-2527. Protein profile is a molecular marker for diversity and phylogenetic analysis of germplasm, including Anabantoidei fish which is abundant in freshwater ecosystem of South Kalimantan. The aim of this research was to analyze genetic diversity and construct phylogenetic relationship among Anabantoidei fish based on SDS PAGE analysis. Protein was extracted from muscle tissue of six species of Anabantoidei fish and precipitated using Ammonium sulfate salt. Soluble protein content was determined using Bradford assay and then separated based on SDS-PAGE method. Genetic diversity and phylogenetic relationship were constructed using PAST software based on UPGMA method. The results showed that the soluble protein of fish muscle can be precipitated optimally in different ammonium sulfate concentration. Based on SDS-PAGE analysis, 59 different protein bands have been separated from gels with molecular weight ranging from 28.15 to 181.61 kDa. On the protein level, the Anabantoidei fish showed high genetic polymorphism (greater than 90%) with 3, 2 and 5 monomorphic bands on non-precipitated, AS-precipitated and combination between non- and AS-precipitated proteins, respectively. The phylogenetic reconstruction also exhibited that the Anabantoidei fish has the unique phylogenetic trees, especially for the combined protein datasets. This information would be useful for freshwater fish conservation and breeding programs.