Scutellariae (Huangqin) is a traditional medicine that is widely applied in China for the treatment of cold and fever, and nowadays research found that it can against pathogenic microorganism. Flavonoids is the main active constituent isolated from Scutellariae and possesses outstanding antibiosis activity. Scutellaria flavonoids displayed antiseptic actions and anti-resistance, including but not limited to destroy bacterial biofilm, suppressed activity of the DNA enzyme, restrain efflux pump and quorum-sensing system. This paper highlights and discusses the antibiosis activity of Scutellaria flavonoids, along with possible mechanisms of its action. hoping to providing basis and evidence for clinical application.