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Samples of wheat naturally contaminated with Fusarium mycotoxins were obtained from fields and mills in Korea and were milled by the Bühler test mill and an industrial-scale mill, respectively. Each of the milling fractions was analyzed for nivalenol (NIV) and deoxynivalenol (DON) by gas chromatography with an electron capture detector and for ZEN by high-performance liquid chromatography with a fluorescence detector. NIV, DON, and ZEN were found throughout all fractions, but ZEN was not detected in break and reduction flour fractions in the industrial mill. The highest concentration of NIV was found in the bran, and DON and ZEN were in the shorts. The lowest concentration of NIV was found in the reduction flour, and DON and ZEN were in the break flour. Milling was not effective in removing NIV, DON, and ZEN from the naturally contaminated wheat, but the effect on its concentration in the samples varied.The determination of concentrations of Fusarium mycotoxins such as nivalenol (NIV), deoxynivalenol (vomitoxin, DON), and zearalenone (F-2 toxin, ZEN) in cereals has been carried out recently in several countries with varied results. Accumulated data have revealed DON to