Bioconjugation of enzymes on coatings based on polydopamine (PDA) layers is an appealing approach to control biological responses on biomedical implant surfaces. As alternative to PDA wet deposition, a fast, solvent-free, and dynamic deposition approach based on atmospheric-pressure plasma dielectric barrier discharge process is considered to deposit on metallic surfaces acrylic-based interlayers containing highly chemically reactive catechol/quinone groups. A biomimetic approach based on covalent immobilization of Dispersin B, an enzyme with antibiofilm properties, shows the bioconjugation potential of the novel plasma polymer layers. The excellent antibiofilm activity against Staphylococcus epidermidis is comparable to the PDA-based layers prepared by wet chemical methods with slow deposition rates. A study of preosteoblastic MG-63 human cell line viability and adhesion properties on plasma polymer layers demonstrates early interaction required for biomedical applications.

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In this study, an efficient methodology, allowing the controlled co-immobilization of two complementary biomolecules, is reported for the production of multifunctional antibacterial surfaces. To promote long-lasting covalent immobilization, metallic surfaces are first coated with a quinone-bearing poly (methacrylate)-based thin film by combining an atmospheric pressure liquidassisted plasma polymerization and a controlled sodium periodate-induced catechol oxidation steps. The influence of the oxidation step on the film morphology and chemistry is investigated using an analytical multitool approach involving atomic force microscopy, ultraviolet, infrared, and X-ray photoelectron spectroscopy techniques. Quartz crystal microbalance with dissipation monitoring (QCM-D) analyses allow the rapid determination of the optimal biomolecule immobilization conditions in terms of kinetics of grafting and biomolecule solution concentrations. In vitro functional assays combined with QCM-D analyses demonstrate promising, dual biologically active coated surfaces.