To study effect of sowing dates on growth and yields of Indian mustard (Brassica junceaL.). Keeping in this view experiment was conducted in Split Plot Design (SPD) with three replications having two factors. First factor comprised of three dates of sowing (15 Oct, 10 Nov. and 05 Dec.) whereas, second factor consist of three Indian mustard varieties viz; Varuna, Narendra rai-1 and Kranti. Results showed that both dates and varieties (10 Nov. and Varuna) superior compare to rest of treatment. However, highest growth attributes (plant height, dry matter accumulation, Days taken to 50% flowering, number of tillers, LAI and yield and yield attributes (No. of siliqua (cm)per plant, length of siliqua (cm) test weight, seed yield(q/ha) grain yield, stover yield, biological yield, and harvest index) was recorded under 10 Nov. and Varuna variety, and oil character. At Lowest yield recorded under dates and varieties at 15 Oct. followed by 05 Dec. and Narendra rai-1 and Kranti. Among treatment, dates and varieties (10 Nov. and Varuna) showed effectively increasing the growth and yield and enhanced the nitrogen content efficiency and oil content and oil yield.
K e y w o r d sSowing dates, Varieties, LAI, No. of siliqua, No. of Branches per plant
Polymerase chain reaction assays of eight yellow mosaic disease (YMD) affected moth bean samples using specific primers indicated the presence of Mungbean yellow mosaic virus (MYMV), Mungbean yellow mosaic India virus (MYMIV) and Horsegram yellow mosaic virus (HgYMV) in two samples (Mo1 and Mo5) and two of these three viruses in remaining six samples. Sequence data analysis of Mo1 and Mo2 revealed the involvement of multiple DNA‐A and DNA‐B molecules in both samples. Further analysis of DNA‐A and DNA‐B molecules confirmed the presence of three viruses viz., MYMIV, MYMV and HgYMV, in Mo1 and of two MYMV and HgYMV in Mo2 samples. Analysis of common region (CR) sequences of components (DNA‐A and DNA‐B) of each virus indicated >85% nucleotide identity confirming them to be cognate and species integrity. The recombination analysis revealed the presence of a total of six putative recombination events in the viruses under study. In a phylogenetic analysis of DNA‐A sequences of the viruses under study with bipartite legume‐infecting begomoviruses and some selected old and new world begomoviruses, MYMIV, MYMV and HgYMV grouped with respective virus species. Similar grouping and clustering were found in the DNA‐B sequences also. The present study reports infection by HgYMV in moth bean and the association of three viruses (MYMIV, MYMV and HgYMV) with moth bean yellow mosaic disease for the first time. The presence of mixed infection of begomoviruses provides opportunities for the exchange of DNA between the species which may lead to the development of new strain/virus species.
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