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In this work, nanoscale luminescent materials dispersed in the air were collected and quantified by the fluorescence spectroscopy. A well-known phosphor; LuAG:Ce3+ was chosen as the model particle due to its strong, measurable and repeatable signal which can easily be excited by the blue light and emits at yellow wavelengths. The ionic liquid modified polymethylmethacrylate based filters were fabricated by electrospinning technique. Samples were collected by means of a vacuum pump from the laboratory environment during the grinding, weighing, transfer, washing, drying and packaging of the phosphorus particles, for different time intervals. The spectrofluorometric method was used for the quantification of the airborne concentration of the nano and microscale dusts. Presented method was also tested in terms of precision, LOD, LOQ, and stability. To the best of our knowledge, this is the first attempt to measure the airborne concentrations of the nano-scale luminescent phosphor particles and can easily be adopted for the quantification of other nanoscale- emitting particles in workplaces. Additionally, the offered design allows miniaturization since it is possible to excite the particles with cost-effective LED based light sources, integrate the system with fiber optics and detect the received optical response by photodiodes.