Aim. The aim of this study was to carry out passportization of cultivars, varieties and hybrids of perennial grass crops: Alopecurus protensis, Agropyron cristatum, Lolium perenne, Lolium miltiflorum, Festuca protensis, Festuca arundinasea, hybrids Festulolium. Methods. The perennial grasses selection was carried out by embryo culture according to methodical recommendations. For the molecular-genetic passportization of perennial grasses varieties the polymorphous primers have been selected – 9 RAPD primers and 15 ISSR primers. Results. Multilocus DNA labeling have allowed to differentiate all the genotypes being studied, to develop and to create the unique profiles for each of them and to calculate the genetic distance of congeniality/distances. On the base of RAPD/ISSR, SCoT spectra the 14 molecular-genetic passports of perennial grass cops have been obtained for the first time. Keywords: grass crops, molecular-genetic passport, DNA, PCR, multipoint mapping RAPD/ISSR spectra.

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Aim. To form the varietal population of festulolium intergeneric hybrids of Festuca arundinacea morphotype. To carry out DNA-labeling of created festulolium hybrid plants and parental forms. Methods. The festulolium intergeneric hybrid’s creation was carried out by embryo culture method from an immature caryopsis by growing on a nutrient medium. For the plant genotypes labeling the multilocus primers associated with coding DNA regions (Start Codon Targeted (SCoT) Polymorphism), SRAP (Sequence-related amplified polymorphism) have been selected. Results. The viable plants of intergeneric festulolium hybrids of Festuca arundinacea morphotype have been obtained. To select the best festulolium biotypes for variety populations with high feed and seed productivity formation. A system for hybrid plants genotypes and their parental forms registration in the form of molecular genetic passports have been elaborated. The genetic passports reflects the allele’s composition in loci associated with DNA coding sequences. Conclusions. The best biotypes with economically valuable traits were selected and included in the further selection process. The molecular genetic passports of hybrid festulolium plants and parental forms were composed. Keywords: festulolium, immature caryopsis, biotypes, DNA, PCR, molecular genetic passports.

Созданы фертильные межродовые гибриды житняка гребенчатого (Agropyron cristatum L.) c райграсом пастбищным (Lolium perenne L.). Проведено мультилокусное ДНК-маркирование житняка с использованием RAPD-и ISSR-праймеров, которое дало возможность дифференцировать все исследованные генотипы. Разработаны и составлены уникальные профили для каждого из них. На основании полученных мультилокусных RAPD/ISSR-спектров для исследованных образцов составлены генетические паспорта.