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Studies were conducted at the dairy complex of «Rus Ltd» (Perm Region). The effect of the use of vitamin-herb flour (VHF) from Rhaponticum carthamoides green mass in the feeding of holstinized black-and-white dairy cows was studied during the period of 12 days before calving and the first 30 days of lactation. The experiment was carried out according to the generally accepted method with the selection of analog pairs. The cows of the control group received a feeding ration consisting of feeds available at the dairy complex. For cows of the experimental groups, the composition of the concentrate part of the diet included VHF from leucea safflower in doses: the first group -4 % (0.4 kg of VHF per head per day), the second group – 10 % (1.0 kg of VHF per head per day). The experimental data of the experiment showed that VHF from leuzea as part of the concentrate part of the diet had a positive effect on the milk productivity of cows during the first 30 days of lactation, as well as on the bioconversion coefficients of protein and energy in the dairy products of cows. During the accounting period of the scientific and economic experiment (the first 30 days of lactation) from cows of the first and second experimental groups there has been obtained milk with natural fat content higher by 55.80±4.90 kg (8.04 %) (p<0.05) and by 99.20±5.80 kg (14.29 %) (p<0.05), respectively, compared to the control group. The gross yield of milk fat and protein for the reference period in cows of the first and second experimental groups was higher by 2.50±0.04 kg and 4.28±0.07 kg, by 2.54±0.16 kg and by 4.01±0.18 kg, respectively, compared with the control group. The cows of the first and second experimental groups had higher coefficients of protein bioconversion into dairy products by 2.98 % abs (p<0.05) and 3.97 % abs, respectively, compared to the control. The energy consumption for milk synthesis in the control group cows was reduced by 163.62 MJ and 349.18 MJ (p<0.05), respectively, in comparison with the cows of the first and second experimental groups, due to their lower consumption of the amount of feed mixture during the accounting period. The cows of the first and second experimental groups also had higher bioconversion coefficients of energy into milk by 2.14 % abs. and by 3.71 % abs. (p<0.05), respectively, compared to the cows of the control group. The cows of the experimental groups turned out to be more responsive to changes in the feeding diet and showed higher results according all the studied parameters.

Aim: To measure the biological activities of extracts of fodder grasses Onobrýchis arenária, Galéga orientális and Rhaponticum carthamoides that are commonly planted in Europe, Middle East and eastern Africa. Methods and Results: Microbial test-systems based on Escherichia coli BW25113that allow measurement of gene expression, growth and survival, biofilm formation (BF) in combination with the standard chemical procedures were used. The extracts studied had radical scavenging and metal-chelating activities and induced expression of antioxidant genes via generation of hydrogen peroxide. However, the extracts did not affect bacterial growth in planktonic cultures but dose-dependently inhibited BF. Conclusions:The most remarkable effects were observed in G. orientalis, a highyielding crop, rich in crude protein and fibres.Significance and Impact of the Study: Taking into account the antibiofilm activities of the extracts, a perspective for decreasing colonization of ruminants' gut with pathogenic bacteria might be suggested in case of feeding with all the grasses studied.

The results of studies of the use of rhaponticum carthamoides for feeding dairy cows are presented. The experiment was carried out on Holstein cows of black-and-white breed in Perm Territory. Vitaminherbal fl our from green mass of rhaponticum carthamoides at a dose of 0.4 and 1.0 kg per head per day in the period of 10-16 days before calving and in the fi rst 30 days of lactation was introduced into the feeding rations of two experimental groups of cows. High sugar content in rhaponticum carthamoides (from 4.15 to 11.2% in dry matter and metabolic energy from 10.87 to 12.12 MJ/ kg) was noted. In the studied material from this plant, the content of the 20-hydroxyecdysone was 0.49% of active ingredients in the dry matter of the product at a rate of 0.25%. The introduction of rhaponticum carthamoides in the diet of feeding animals contributed to the increase in the intensity of metabolic processes in the body. A gradual increase in cholesterol levels in all experimental groups was noted. The most signifi cant growth was observed in cows of the second experimental group by 0.82 mmol / l or 29.50% (p <0.01). Compared with the fi rst experimental group, the increase was 0.13 mmol / l or 5.2%, and the control group – 0.36 mmol / l or 14.17%. Stimulation of metabolic processes in the body of cows of the experimental groups had a positive impact on reproduction, which was manifested in the reduction of the service period duration, compared with the control group.

В последние годы в Пермском крае прекратилось известкование кислых почв, недостаточно вносятся минеральные и органические удобрения. Всё это постепенно привело к снижению плодородия почв. Избежать или снизить влияние негативных явлений можно за счёт использования многолетних трав. Перспективной фитомелиоративной культурой является эспарцет песчаный (Onobrychis arenaria (Kit.) Ser.), который ранее в Пермском крае не возделывался, но встречается в естественных фитоценозах ряда районов края. Целью настоящей работы является поиск и обобщение научной информации по морфо-биологическим особенностям и агроэкологической роли эспарцета песчаного в земледелии. В статье приведён литературный обзор биологических особенностей этой культуры: азотфиксации, накопления пожнивно-корневых остатков, формирования корневой системы эспарцета песчаного в различных регионах его возделывания. Для сравнения приведены экспериментальные данные ряда исследователей, полученные в Пермском крае при изучении клевера лугового (Trifolium pratense L.), люцерны изменчивой (Medicago×varia Mart.), донника белого (Melilotus albus Medik.). Эспарцет песчаный за счёт корневых выделений способен усваивать из глубоких горизонтов почвы труднодоступные для других культур кальциевые и фосфорные соединения и обогащать ими верхний горизонт почвы, то есть он может выступать в роли биологического мелиоранта. На опытном поле Пермского НИИСХ эспарцет песчаный изучался с 2011 по 2019 год и зарекомендовал себя как перспективная кормовая культура. Изучение его особенностей с целью разработки технологий возделывания и использования целесообразно продолжить и расширить. Over the last years, liming of acidic soils stopped in the Perm Territory; mineral and organic fertilizers are not applied sufficiently. All this gradually led to a decrease in soil fertility. It is possible to avoid or reduce the influence of negative factors by planting perennial grasses. Hungarian sainfoin (Onobrychis arenaria (Kit.) Ser.) is a promising phytomeliorative crop for the Perm Territory. It was not previously cultivated there, but it is found in natural ecosystems in a number of locations. The investigation aimed at reviewing morphology, biology and ecological value of Hungarian sainfoin. The article deals with its biological features: nitrogen fixation, accumulation of crop residues as well as root system formation in various regions. To compare sainfoin with other crops in the Perm Territory, the article provides the experimental data on red clover (Trifolium pratense L.), alfalfa (Medicago×varia Mart.), and white melilot (Melilotus albus Medik.). Due to root secretions, Hungarian sainfoin is able to assimilate calcium and phosphorus from deep soil that are unavailable for other crops. So, the crop can enrich the upper soil layers with these elements, being a biological ameliorant. Studied during 2011–2019 on the trial field of the Perm Research Institute of Agriculture, Hungarian sainfoin was shown to be a promising forage crop. Its further investigation as well as development of efficient cultural practices is of high value.