Long-term changes of wolf feeding habits have been recorded in several parts of its European range and were related to the increase of wild ungulate populations. An increased use of wild prey often is linked to a decrease of predation on livestock, and consequently it can reduce wolf-human conflict and make for easier predator conservation. We analysed wolf diet from scats collected from 2007 to 2012 in a 927.3-km 2 area of the Northern Apennines (Northern Italy) to compare the present diet to that recorded in the late 1980s when the area was colonised by wolves, and to examine short-term changes of wolf feeding habits together with annual and seasonal variations of wild and domestic ungulate selection, possibly related to the increase of wild ungulate abundance. We calculated the availability proportions of wild ungulate species from the presence signs recorded on itineraries and those of livestock species from the data on farms of veterinary services. We identified 17 kinds of prey, pooled into six categories, of which wild ungulates (70%) and livestock (20%) were the most important. During the study period, we observed a reduction in the diet breadth of wolves, to a greater use of wild ungulates, depending on the increase in the availability of wild prey species. Significant seasonal variations were observed in the trophic habits of the wolf, but generally there was a wide use of wild ungulates, especially wild boars. Livestock was mainly used in summer, which corresponds to the period of greatest presence of domestic ungulates on the pastures. Considering the study period, a fast response of wolf feeding habits to the increase of wild ungulate species resulted, together with changes in selection patterns. The change in diet towards a greater use of wild ungulates is an important step in the recovery and conservation of the wolf.