Methanolic and aqueous leaf extracts of Chrysophyllum albidum (African star apple) and Garcinia kola (bitter kola) were studied for in-vitro microbial activity using the disc diffusion technique. The aqueous and methanolic leaf extracts of Chrysophyllum albidum showed antimicrobial activity against Escherichia coli, Staphylococcus aureus and Candida albicans but showed no antibacterial activity against Klebsiella pneumonia. The methanolic leaf extract of Garcinia kola inhibited the growth of Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia while the aqueous extract of the leaf inhibited the growth of Staphylococcus aureus and Escherichia coli. Both methanolic and aqueous leaf extracts of the plant showed no antifungal activity against Candida albicans. The minimum inhibitory concentrations of the leaf extracts of Chrysophyllum albidum ranged from 12.5 mgL-1 to 25 mgL-1 while those of Garcinia kola ranged from 25 mgL-1 to 50 mgL-1. The results obtained suggest that the leaves of these plants can be used in treating diseases caused by the test organisms. The further investigation on the crude extracts would characterize bioactive components of the leaves of Chrysophyllum albidum and Garcinia kola.
In the quest for a sustainable nematode control strategy, alkaloids of physic nut (Jatropha curcas) plant parts were evaluated for their nematicidal control on the root-gall nematode disease of Okra and its mucilaginous property and nutrient composition in 2019 and 2020 cropping season. The experiment was a 3×6 factorial experiments arranged in Completely Randomized Design with five replications in the screen house. Alkaloids extracted from the seeds, leaves and roots of physic nut plants were applied separately at 0, 1, 2, 3, 4 and 5 ml / potted okra plant inoculated with 1,200 infective (J2) larvae of M. incognita. Results showed that 5 ml of Root and Seed Alkaloids application significantly (P=0.05) reduced root-gall nematode infection to a gall index of 1 and 1.2 (rarely galled) when compared to the untreated control (4 -severely galled) plants thereby achieving more than 100 percent increase in mucilaginous property of okra over the control for both years of study. Moisture content, fat, calcium and sodium also increased more than the untreated control at 5 ml of root and seed alkaloids. Phosphorus and nitrogen contents were on the other hand rather enhanced by the infection. Neither Okra nor root-gall nematode infection affected ash, magnesium and potassium contents of the fruits. Nevertheless, bioactive active constituent of Physicnut plant parts such as root and seed alkaloids can thus be developed as eco-friendly non-synthetic nematicide for improved yield.
This study is focused on the management of phytophthora blight, a fungal disease of “pigeon pea” by the application of some aqueous extracts of neem plant parts. The aim of the study was to manage the phytophthora blight of pigeon pea using the plant parts extract of neem. The study was conducted in the year, 2021, at the Demonstration Farms (both field and laboratory) of the Department of Crop Science and Technology, School of Agriculture and Agricultural Technology, Federal University of Technology, Owerri, Imo State, Nigeria. The experiment was designed as Randomized Complete Block Design (RCBD) with five replications. Data on pigeon pea growth parameters and disease incidence and severity, were statistically analyzed by the use of Analysis of Variance (ANOVA) software as outlined by Wahua. The means were separated for difference using the Fisher’s Least Significant Difference (FLSD). Results indicated that aqueous extract of neem seed application caused the tallest Pigeon pea height (103.30 cm), the highest number of pigeon pea leaves (99.40), the lowest phytophthora blight disease incidence (11.00%) and also the lowest phytophthora blight disease severity (1.00).
Background: Raw palm oil mill effluent is toxic effluent capable of posing serious threat to plants when discharged to the environment. In developing countries, the untreated effluent is often discharged to the surrounding land due to high cost associated with its treatment. The aim of the current study was to evaluate the impact of palm oil mill effluent treated with locally available material on the growth of maize. Methods: The bunch ash obtained from local material by burning de-fruited oil palm bunch was used for the treatment. The experiment was consisted of eight treatments of concentrations of palm oil mill effluent viz 1.0%, 2.0%, 3.0%, 4.0%, 5.0%, 6.0%, 7.0% and 8.0%. The treatment was carried out on potted maize plants with six replications. Four kilograms of soil was weighed into each pot. Six pots were labelled as control, with untreated palm oil mill effluent added to them. Eight other groups consisting of six pots each were treated with 1.0%, 2.0%, 3.0%, 4.0%, 5.0%, 6.0%, 7.0% and 8.0% concentrations of the effluent. The maize seeds were planted one in each pot to avoid overcrowding. The pots were transferred to greenhouse and each pot was moistened daily with the specified concentration of the palm oil mill effluent. The growth rate, plant height and leaf length of the maize plants were thereafter determined. Result: The 8.0% concentration of bunch ash in the effluent had the highest expected effect on all the parameters whereas the control gave the lowest expected effect. The effects increased as the concentration of bunch ash in the effluent increased, indicating that increased concentration of bunch ash recorded significant increase in growth rate, height and leaf length of maize. The analysis of variance for the obtained data showed that the effects of the different concentrations were significantly different. This study provides an alternative and cost effective method of ameliorating the toxicity of palm oil mill effluent to plants.
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