There are no detailed studies on the nutritional and microbiological characteristics of honey produced in Ogun State in the Southwestern part of Nigeria. This paper investigated these components in honeys produced from different parts of this state. A total of 10 honey samples per year were collected for the years 2008 -2010. These were separately analyzed for their physical properties, nutritional and microbiological components in the laboratory. The results of the physical properties showed that honey samples obtained from Ago-Iwoye has the lowest pH of 3.48, which was significantly different from the pH values of 5.06, 5.21 and 4.06 obtained from honey samples from Abeokuta, Ibefun and Ilisan honey samples respectively. There was significant difference in moisture contents of honey samples obtained from Ogere (16.19 %), Otta (19.14 %) and Ijebu-Ode (18.21 %), while the percentage ash contents of 0.78 % obtained from honey samples collected from Abeokuta was not significantly different from the value of 0.75 % obtained from Ago-Iwoye honey (P ≤ 0.05). However, the value of 1.11 mg 100 g -1 for hydroxymethylfurfural obtained from Ago-Iwoye honey samples was not significantly different from the value of 0.32 mg 100 g -1 in honey samples obtained from Sagamu. The value for glucose ranged from 18.42 -30.16 g 100 g -1 , while fructose sugar varied between 25.42 -38.21 g 100 g -1 . Minimum protein value of 0.02 % was obtained from Ijebu-Ode honey, while the maximum of 0.51 % was obtained from honey samples from Ilisan. Results of the elemental nutrient showed that potassium was the most abundant element in honey samples with the range value of 14.78 -17.42 mg 100 g -1 followed by calcium, which varied from 2.13 -11.25 mg 100 g -1 . However, result of microbiological properties showed that the total plate count varied from 0.2 -3.4 cfu g -1 , whereas total coliforms were not detected in honey samples collected from Ago-Iwoye, Otta, Ibefun, Ife and Sagamu. Clostridium spp, Bacillus spp and yeast were also detected at low count in all honey samples investigated. Moreover, honey samples obtained from different parts of Ogun State were found to be contaminated with Pseudomonas spp., Xanthomonas spp., Bacillus spp., E. coli and Clostridium spp, while fungi spores intercepted were Pennicillium oxalicum, Aspergilus niger, A. Flavus and Fusarium oxysporum at different levels and counts.