The laser excited fluorescence-line-narrowed spectrum of DNA modified with (+/-)-r-7,t-8-dihydroxy-t-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE), the ultimate carcinogenic metabolite of benzo[a]pyrene (BP), has been obtained in a water-glycerol-ethanol glass at 4.2 K. The spectrum was well resolved and highly characteristic of the chromophore. Comparisons were made between the spectrum of this modified DNA and the isolated deoxyguanosine-BPDE adduct and a series of other 7,8,9,10-tetrahydro-BP (THBP) derivatives. 9-Hydroxy-BP 4,5-oxide, which is also involved in the binding of BP to DNA, and THBP have very similar conventional broadband fluorescence spectra. However, the fluorescence-line-narrowed spectra of their derivatives were readily distinguishable either as individual components or as mixtures.

No abstract

No abstract

The conversion of G[3H]benzo(a)pyrene to water-soluble material and to DNA adducts was determined in peripheral blood monocytes of 52 healthy male and female volunteers and 27 patients with lung cancer. Active smokers converted more benzpyrene to DNA-bound material than nonsmokers, but did not form significantly more water soluble material. Monocytes of lung cancer patients clearly formed more water soluble material than cells of the control group, and slightly more DNA adducts when only males are compared. This enhanced BP conversion of lung cancer patients is independent of smoking habits.

No abstract