The genotypic characteristics of the Sapphire genotype (SG) pigs and their hybridization with the вoars Landras (L) and Duroc (D) of 2-breed (SGхL) and 3-breed [(SGxL) xD] hybrids by genes: FABR-H, FABR-D, Ms4r, LEP, ECR, RYR-1 was investigated. In SG animals, the frequency of the ECRGG genotype, which is increased in comparison with 2- and 3- breed hybrids, is 0.41-0.54. Two-breed hybrids are characterized by a higher frequency of the genotype FABP HH (by 0.22-0.27) and FABP dd (by 0.27-0.39) than SG and three-breed. The peculiarity of the 3-breed hybrids (SGxL) xD is the higher frequency of the homozygous genotype Ms4r AA (by 0.24-0.34), and the lower frequency of the heterozygous Mc4r AG (by 0.19-0.43) compared with pure SG and 2-breed hybrids. The polymorphism of the LEP and RYR-1 genes in the compared groups of pigs is mainly represented by the desired genotypes LEPTT (71-75 %) and RYR-1NN (71-86 %). The indices of genetic similarity between the SG and hybrids were determined. The most significant differences were found between 2-breed and 3-breed hybrids (0.7634 ± 0.0613), which can be explained by the presence of blood of the breed D. General homozygosity (GH ) in pigs SG was 24.89 %, in 3-breed hybrids - 14.6 %. The genotypic structure of stress-resistant and stress-sensitive animals and associative connection of genotypes with energy of growth are revealed. Piglets with genotype FABPHH, resistant to stress, during the growing period surpassed their peers with the FABPHh and FABPhh genotypes by an average daily gain of 81-139 g, sensitive to stress by 134 g. Animals with the FABPDD genotype, stress-resistant, had the highest energy of growth (593 g), which was higher than in peers with the homozygous genotype FABP Ddd by 91 g and heterozygous FABP DDd by 157 g. Among the piglets sensitive to stress, individuals with the LEPTT genotype had a higher growth energy of 87 g compared to heterozygous LEPTC. Stress-resistant pigs with the RYRNn genotype outperformed homozygous peers of RYRNN in this indicator by 95 g. Among the carriers of the Mc4r and ECR genotypes, no differences in growth energy of the pigs were detected.
Three experiments were carried out to improve the meat productivity of pigs of Large White (LW) breed of russian selection by different variants of crossing with the Yorkshire (Y) breed of canadian selection. There were no significant differences between the groups in terms of reproductive qualities. Two-breed pigs (LW x Y) grew more intensively and the live weight of the piglet at the 30 days was 8,7 kg, which is higher than in the other groups with a significant difference (P <0,05). When the live weight of 100 kg was reached, the вackfat thickness of the fat over 6-7 thoracic vertebrae was 26,3 mm compared to 30,7 mm in purebred pigs of the LW of the breed (P <0,001), the area of the muscular eye was respectively 45,1 cm 2 and 30,6 cm 2 (P <0,001). In the fourth generation of reproductive and absorbent crossing of pigs of LW and Y of breeds, the thickness of the fat on the carcasses of pigs was 21,2 mm (Р < 0.001), which is 30-39 % less than for the LW carcasses, the area of the muscular eye was 44 mm. In three-breed hybrids (LW x D) x L, the age of reaching 100kg was less by 12,5 days (P <0,01), the thickness of the fat was 23,6 mm, that is, it was 11,4 mm less (P < 0,001) in comparison with the control group. The replacement pigs obtained from the absorbent crossing (LW x Y) x Y showed the best results for the вackfat thickness - 19,2 mm, but they were inferior to the purebred Y (13,0 mm) (P <0,001). In the sows of this combination, 11,6 piglets were born alive, the number of piglets at weaning was 10,6, the weight of the nest in the 30-day was 78,7 kg. Differences in the frequency of occurrence of certain antigenic factors of blood in animals of different combinations of breeds are revealed. In animals that have the EAE allele edf, a higher growth rate is revealed, but thicker the backfat.
Background: The studies were carried out on quails, in the feed of which fat of various composition was added. Objective: to establish the effect of adding fat from pigs of different breeds and vegetable oil to quail feed on their growth, productivity and characteristics of lipid metabolism. Methods: chickens of three groups of 60 individuals each were fed with vegetable oil (control) and fat of two pig breeds sharply differing in the chemical composition of fat - Kemerovo (К) and Pietrain (P) up to 23 weeks of age (3.0 - 3.5% of the mass of the diet). At 8 and 23 weeks of age, 10 individuals from each group underwent biochemical blood tests. Results: The fastest growing quails that received fat of breed K. The level of total cholesterol (TC), during the period of the experiment, of the quails significantly decreased, including those receiving vegetable oil - 1.49 times the fat of breed K - 1, 58 times, the fat of breed P - 1.32 times. The atherogenic index (AI) turned out to be significantly higher (P <0.01) in quails fed breed P fat, containing a high level of TC and rich in PUFA (0.93 conventional units versus 0.69 in birds receiving fat of breed K and 0, 57 - in the control group). Conclusion: The obtained data indicate that fat of breed K is more useful for animal nutrition and may be a priority in human nutrition.
The authors presented the results of experimental studies on the stress-resistant of pigs. The first group is a breed created in Sapphire Ltd. This breed is a breeding group (BG) in purebred breeding and their mixtures in two- and three-breed combinations with Landrace (L) and Duroc (D) boars under conditions of industrial farm technology in Siberia. Two methods assessed stress-resistant of piglets of different breed groups. The first method is “weaning crisis”. The second method is a com-parison of cortisol levels in the blood. Three-breed weanling piglets (SGxL)xD turned out to be the most stress-sensitive. Stress-resistant piglets had an effect on their growth during the rearing period. During this period, stress-resistant animals of all breed combinations had higher average daily gain than stress-sensitive animals (P < 0.001). Stress-resistant animals of the breeding group (SG) showed an average daily growth of 547.5 g during the fattening period. Also, the stress-resistant animals of the breeding group reliably surpassed the stress-sensitive pigs by 461.4 g (P < 0.01), the two-breed pigs by 455.9 g and 404.7 g and the three-breed pigs 451.8 g and 419.2 g, respectively. There was a statistically significant advantage in the indices of the average daily gain among the purebred young-sters of the breeding group (SG) (543 g) compared to the two-breed pigs (447g) and the three-breed pigs (402g), i. e., by 17.8 % and 26 % at P < 0.001. The authors found that the EAAcr/- genotype in stress-sensitive pigs was more common than EAA-/(0.71 ± 0.07 vs 0.48 ± 0.09). Stress-resistant pigs of the breeding group (SG) with EAE edg/edf blood group genotypes were characterized by increased growth intensity and reliable superiority over stress-sensitive pigs. The authors believe that these genotypes can be accepted as preliminary candidates for genetic markers of stress resistant. Blood cortisol levels appeared to be unrelated to stress-resistant compared to the “weaning crisis” method. This relationship (blood cortisol level with stress-resistant) applies to all studied breed combinations, both individually and as a whole.
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