The problem of the medical application of non-invasive (The specially devised multifunctional laser diagnostic system “LAKK-M” was used as a diagnostic instrument in the majority of studies in MONIKI, allowing a combination of methods, such as non-invasive medical spectrophotometry in the form of laser fluorescence spectroscopy, laser Doppler flowmetry, tissue reflectance oximetry, etc. The system was used in both the experiments on laboratory animals, and for treatment of patients in different clinics of the institute.Within the last decade, extensive scientific data has been obtained which has opened up the possibility of using non-invasive medical spectrophotometryMethods of complex non-invasive medical spectrophotometry appear to be an efficient tool in practical medicine for differential diagnostics of a number of diseases and pathologies, as well as for monitoring and prediction of the treatment outcome. At the same time, they also create an important perspective for gaining novel and fundamental knowledge about the blood microcirculation system which was not available earlier due to the absence of the diagnostic technology allowing information to be obtained

This report, as a continuation of 8-years research on the problem of noninvasive clinical fluorescence diagnostics efficiency, discusses a hypothesis of influence of a chronic hypoxia state in soft alive tissues on the intensity of a laser-induced endogenous porphyrins' autofluorescence in a red region of optical spectra. Earlier this hypothesis was proposed on the basis of analysis of fluorescence activity for erosive-ulcerative impairments of the upper part of a gastrointestinal tract (SPIE Proc., vol. 4613, 2002. -p.286-294). Today the hypothesis additionally is confirmed by means of observation after patients with another illness and by means of analysis of some well-known literature data. An authors' methodology of clinical trails to verify the hypothesis using an up-to-date noninvasive fluorescence diagnostic technique is presented as well. Both theoretical reasons and all new clinical data show that the chronic hypoxia state can be one of the major factors of appearance of a large and abnormal laser-induced autofluorescent signal from biotissues in the spectrum range 600-800 nm, which is associated with abnormally high accumulation of endogenous porphyrins in the tissues. So, the noninvasive autofluorescent diagnostic technique could be a powerful tool to estimate in vivo a chronic hypoxia condition in soft biotissues. For that purpose a classification of chronic hypoxia levels versus in vivo autofluorescence contrast coefficients in tissues is proposed as well.

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