Central odontogenic fibroma (COF) represents a rare group of benign odontogenic tumor, while dentigerous cyst (DC), on the other hand, is the odontogenic cyst that encloses the crown of an unerupted tooth. A combination of COF and DC is rare and not reported in literature till date. The aim of this paper is to report a case of COF occurring concurrently with DC. The simultaneous occurrence of COF and DC raises the question of whether it is a collision tumor or their simultaneous occurrence is just a coincidence. In our case, the probable cause of simultaneous occurrence might be that COF would have displaced 23 resulting in the formation of DC.

Introduction: Tissue processing involves transition of the biopsy tissue in graded concentration of various chemicals to make the tissue amiable for sectioning. The entire process takes 2–3 working days before a microscopic slide is ready for diagnosis. In order to shorten the turnaround time, rapid tissue processing method using methyl salicylate was developed. Aim: The aim of this study is to develop a rapid tissue processing technique using methyl salicylate as a clearing agent and to compare it with routine tissue processing technique. Materials and Methods: A total of 70 tissue specimens were cut into two equal halves. One each was processed by routine processing technique (RoPT) and rapid processing technique (RaPT). Tissue specimens were measured before and after processing. Quality of staining and cellular-level shrinkage were observed and scored for specimens. Statistical analysis using Welch's unequal variances t -test was performed. Costs of chemicals in both the techniques were compared to see the cost-effectiveness of RaPT. Results and Conclusion: Outcomes of both the processing techniques were comparable with statistically not significant P values for all the parameters. Hence, the results of RaPT technique are satisfactory, and the use of this technique may prove beneficial to pathology laboratories.

Introduction: Salivary matrix metalloproteinase-9 (MMP-9) is an established diagnostic biomarker for detection and prognosis of oral cancer. Objectives: The current study aimed to estimate salivary MMP-9 in oral leukoplakia and oral submucous fibrosis (OSMF), which are considered as oral potentially malignant disorders (OPMDs). Material and methods: A comparative observational study of 60 subjects was conducted, including 20 patients diagnosed with OSMF (group A, n = 20), 20 diagnosed with oral leukoplakia (group B, n = 20), and 20 as healthy controls (group C, n = 20) according to standard criteria. Unstimulated salivary samples were collected for evaluation of MMP-9 by enzyme-linked immunosorbent assay (ELISA). One-way analysis of variance (ANOVA) test was applied for comparison of groups. Bonferroni-adjusted one-way ANOVA test was performed for intra-group comparison. Results: Descriptive statistics of the data revealed that mean age of study participants in group A, group B, and group C were 50.30 ± 9.96 years, 46.70 ± 12.59 years, and 33.30 ± 4.70 years, respectively. Mean salivary MMP-9 level in OSMF, oral leukoplakia, and control group were 9.42 ng/ml, 10.59 ng/ml, and 2.96 ng/ml, respectively, with a p-value < 0.001 between OSMF and oral leukoplakia when compared with controls. Conclusions: A significant difference was noted in salivary MMP-9 levels among patients with OPMD's compared to controls. A slightly higher value of MMP-9 was observed in oral leukoplakia. Estimation of salivary MMP-9 level is a non-invasive and a reliable biomarker in diagnosis of OPMD's and in accessing their malignant transformation.

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Background: Although recurrent aphthous stomatitis (RAS) is the most common oral mucosal disease, the etiology remains poorly understood till date. In addition, RAS lacks definitive therapeutic options, and hence, it becomes even more important to know the etiological factor so as to relieve the symptoms and render treatment effectively. Recently, late bedtime has been shown to be associated with RAS. Due to prolonged duty hours along with lengthy learning and training periods, late bedtime is a frequent observation among health-care professional students. However, this is a less explored field in the previous studies on RAS. Aims: This study aimed to evaluate the role of bedtime in the causation of RAS among health-care professional students. Methods: This present cross-sectional survey was carried out among medical, dental and nursing students studying in our medical campus, using an anonymous, self-administered questionnaire consisting of 18 items. The collected data was statistically analyzed and interpreted. Descriptive analysis and Chi-square tests were employed to assess the association of RAS with various variables. Results: Among a total of 1111 students who participated in the study, 39.8% ( n = 442) experienced RAS. Factors such as history of digestive tract disease, family history and stress showed a positive correlation with RAS. Interestingly, both during examination and nonexamination period, RAS was more prevalent among students with bedtime >11pm than in students with bedtime <11 pm. Conclusion: The study indicates that late bedtime is an independent risk factor for RAS and thus clinicians should focus on recommending a healthy lifestyle with adequate sleep to patients with RAS. This would benefit patients with RAS, especially health-care professional students, and could probably contribute to long-term remission in RAS.