Lusková V., M. Svoboda, J. Koláfiová: The Effect of Diazinon on Blood Plasma Biochemistry in Carp (Cyprinus carpio L.). Acta Vet. Brno 2002, 71: 117-123.The aim of this study was to evaluate the effect of diazinon [0,0-diethyl-0-(2-isopropyl-6-methylpyrimidin-4yl) phosphorothioate] on common carp (Cyprinus carpio L.). The effect was assessed by comparing the biochemical blood plasma profiles of a control group and a group exposed to the effect of the pesticide Basudin 600 EW (containing 600 g.l -1 diazinon as the toxic substance). The activities of selected enzymes, metabolite concentrations and electrolytes were measured in 15 specimens of controls, K 1-2, and in 16 specimens, K 1-2 , exposed for 96 h to the effects of Basudin 600 EW at a concentration of 32.5 mg.l -1 . Compared with the control group, a significant decrease of cholinesterase (p < 0.01) and lactate dehydrogenase (p < 0.05) was ascertained in the experimental group. The values of alanine and aspartate aminotransferases, creatine kinase, alkaline and acide phosphatases were comparable in the experimental and control groups. The total protein and lactate concentration were significantly lower (p < 0.05) in the experimental group, compared with the control group. On the contrary, glucose concentration in the plasma of the experimental group was significantly higher (p < 0.01) than in that of the control group. The experimental group showed a significantly higher (p < 0.05) concentration of plasmatic natrium and potassium, and a significantly lower (p < 0.05) concentration of plasmatic calcium and phosphorus, compared with those in the control group. The above results of examinations of the biochemical blood plasma profile indicate a marked neurotoxic effect of diazinon in fishes.

The goal was to assess an effect of diazinon [0,0-diethyl 0-(2-isopropyl-6-methylpyrimidin-4yl) phosphorothioate] on common carp (Cyprinus carpio L.). The effect was assessed based on results of acute toxicity tests and on a comparison of results of haematological examination of a control and an experimental group exposed to Basudin 600 EW pesticide preparation (active substance 600 g.l -1 of diazinon). The acute toxicity test lasting 96 h was performed semistatically on common carp juveniles. Examination of erythrocyte and leukocyte profile was performed on 15 control and 25 experimental specimens of one-to-two-year-old common carp after 96 h of exposure to Basudin 600 EW in concentration of 32.5 mg . l -1 . The 96hLC50 value of Basudin 600 EW for common carp juveniles was 26.7 mg . l -1 . The experimental group of one-to two-year-old common carp showed significantly lower values (p < 0.01) of erythrocyte count (RBC), haemoglobin content (Hb) and haematocrit (PCV) compared to the control group. Values of MCV, MCH and MCHC were comparable in both groups during the study. In contrary, there was a significant decrease in leukocyte count (Leuko) (p < 0.01), as well as in both the relative and absolute lymphocyte count (p < 0.01) and a significant increase in both the relative and absolute count of developmental forms of neutrophile granulocytes: myelocytes (p < 0.01) and metamyelocytes (p < 0.05) in the experimental group. Relative and absolute count of monocytes and both the band-and segmented neutrophile granulocytes was comparable in both groups during the study. The diazinon-based Basudin 600 EW pesticide preparation was classified among harmful substances for fish. Changes in values of both the erythrocyte and leukocyte profile after exposure to diazinon-based preparation may be refered to disruption of haematopoiesis as well as to a decrease on non-specific immunity of the fish.Organophosphorous pesticide, acute toxicity, erythrocyte profile, leukocyte profile

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