Sixteen multifarious cows were selected on the basis of average daily milk yield and stage of lactation; they were divided into four groups (four cows in each group) with parity within each group. These cows were fed 10gm, 15gm and 20gm probiotics just before evening milk. The multi-strain probiotic used were containing four strains consist of bacteria and fungi namely Lactobacillus acidophilus, Saccharomyces cerevisiae, Saccharomyces boulardii and Propionibacterium frendenreichii. It was found that, the use of probiotics proved to be effective in increasing milk production of lactating cows. Milk fat, milk protein and SNF content tended to be higher in cows supplemented with probiotics preparations. The appropriate level of 20 gm probiotic per day per animal was found effective. The economic advantage of probiotics depends on its relative prices and on the levels of milk production of the cows.