Porcine circovirus type 2 (PCV2) has been demonstrated to be the causal agent for postweaning multisystemic wasting syndrome (PMWS) and porcine dermatitis and nephropathy syndrome (PDNS). This report describes the first detection of PCV2 and associated diseases in a Romanian swine herd located in Transylvania. The clinical signs, pathological and histopathological changes observed in affected pigs were similar to those previously described for PDNS and PMWS. Polymerase chain reaction and in situ hybridisation were used for the detection of PCV2 nucleic acids from tissues and serum samples. Complete PCV2 genomes of both PMWS and PDNS cases were sequenced and analysed, and by comparing them with each other no genomic differences could be detected. The sequence analysis showed that the Romanian PCV2 was closely related to PCV2 identified in France and in Hungary.

Lipid-rich invasive human breast cancer is a rare enigmatic entity among special types of infiltrating duct carcinoma. Our paper reports a lipid-rich mammary carcinoma in a female cat with the gross, microscopic and immunohistochemical description of the tumor. A 13-year-old intact adult female, mixed-breed cat was presented by the owner to the Laboratory of Pathologic Anatomy from the Faculty of Veterinary Medicine ClujNapoca, Romania. A complete necropsy examination was performed in our laboratory. The tissue samples were collected and processed by paraffin technique for further histological, histochemical, immunofluorescence and immunohistochemical examination. During the necropsy examination, a subcutaneous mass was discovered on the chest. Several variably sized, well-demarcated neoplasms were noted in the right axillary lymph node, right thoracic wall, pleura, lungs, liver, spleen and kidney. Histologically, the cells frequently formed tubuloacinar structures. The morphology of the described tumor showed features of a poorly differentiated mammary carcinoma. Numerous tumoral cells were large and polygonal, with abundant cytoplasm that showed foam-like cytoplasm. The tumoral cells contained either multiple small or large and solitary vacuoles that pushed the nucleus to the periphery of the cell. Intracytoplasmic vacuoles of the neoplastic cells were positive for Oil-Red-O and negatively with Periodic Acid-Schiff. As for immunofluorescence/immunohistochemistry, nonvacuolated and vacuolated neoplastic cells were positive for cytokeratin and negative for vimentin. Histochemical and immunohistochemical analysis support a diagnosis of lipid-rich mammary carcinoma. This is the second reliable record of a lipid-rich mammary carcinoma in female cat and the first one with internal metastases.

Background: Mucous glands from the gastroduodenal junction are briefly represented by glandular cells in the stomach and by Brunner’s glands in the duodenum. The aim of the study was to describe the main histological/histochemical features of the gastroduodenal junction in guinea pigs and chinchillas. Results: The material was represented by tissue samples collected from the stomach and duodenum processed by paraffin technique. Histological sections were stained by Goldner’s trichrome stain, while mucous content was identified by Periodic acid–Schiff (PAS) stain (for neutral mucosubstances) and Alcian blue stain (for acid mucosubstances). In guinea pig, the pyloric glands were positive for both mucus types, whereas in chinchillas a low amount of mucin was identified. The Bruner glands, in guinea pigs displayed a weak reaction for the both mucin types. In chinchillas, the Bruner glands showed a significantly higher amount of PAS and Alcian blue-positives mucosubstances types. As observed, a higher amount of neutral mucus was identified in the pyloric glands in guinea pigs comparing to chinchillas. Contrariwise, chinchillas displayed a suggestively higher amount of mucus in Bruner glands comparing to guinea pigs. Conclusions: Concluding, this is the first report describing the comparative features of the mucous glands in the two rodents, with detailed histological and histochemical features.

The materials used for major sinus lift varies from autogenous or allogenous bone, to animal origin (xenograft) bone or synthetic (alloplastic) materials. A combination of bone substitutes, alloplastic graft with allogenic or xenogenic bone seems to give satisfactory results in terms of new bone formation and the long-term implant success rate. On the other hand, using PRP (Platelet Rich Plasma) mixed with bone substitutes seems to enhance graft vascularization and osseous regeneration, as well as faster healing. The novel sinus lift technique with PRGF (Plasma rich in Growth Factors) has demonstrated significant development of new bone, minimizing any complications in the event of Scheneiderian membrane perforation. The present study compares two clinical cases where major sinus lifts were performed using as regenerative material xenograft bone and porous titanium mixed with PRGF.