The current experiment was conducted in the Research Field of the Department of Horticulture, SHUATS, Prayagraj, from November 2021 to March 2022. The goal of the experiment, which used a Randomized Block Design (RBD) with eight treatments and three replications, was to determine which variety will grow best in Prayagraj agroclimatic conditions. It is determined from the current experimental results that the eight treatments in the American beauty variety under consideration demonstrated substantial diversity in all the parameters noted. to investigate "The Effect of NPK and Nano Fertilizer on Growth, Yield, and Quality of Gladiolus (Gladiolus grandiflorus L)". The best growth in plant height (116.22cm), number of leaves (14.0), number of shoots 50% sprouting (19.73), spike length (72.33 cm), and first spike emergence were all recorded (76.8), days it took for the first flower to bloom (118.44 ) 50% florets (106.88 ), spike weight (93.81 gm), and first and second floret flower diameter (6.27, 6.01 cm) Corms per plant (2.33 gm), corms per plot (20.31), vase life (11.5), number of spikes (1.83), and number of florets(12.77) compared to other treatments, T5 nano iron 0.25g/l offered the best results in terms of gladiolus growth, yield, and quality. The highest T5 nano iron 0.25g/l net income was (1563017) Rs./Ha, the highest gross return was (2187165) Rs./Ha, and the highest BC ratio was (1:2.5).