The proposed work involves High Performance Liquid Chromatographic – Diode Array Detector method for estimation of escitalopram oxalate and flupentixol dihydrochloride in combination. The analytes were resolved on Waters C18 Xterra column (50 × 4.6 mm id, particle size 3.5 μm) with mobile phase composition of water (containing 0.2 % triethylamine pH adjusted to 2.5 with orthophosphoric acid): acetonitrile: methanol (62:28:10 % v/v/v). The flow rate kept was 1.0 mL/min opting isocratic mode and eluents were tracked down at 230 nm. The retention times for both the drugs were 1.38±0.21 and 2.98±0.41, respectively. Standard addition method was employed for the analysis of flupentixol dihydrochloride. ICH guidelines were followed to validate the method. The method assured linearity in concentration range of 50-150 μg/mL and 2.5-7.5 μg/mL for ESC and FLU, respectively. The method assured high degree of precision and accuracy. The method was proved to be robust by assessing robustness parameters.

A high performance thin layer chromatographic method has been reported for estimation of losartan potassium with hydrochlorothiazide in combination. The analytes were resolved on precoated silica gel 60 F254 TLC plates prewashed with methanol using solvent system composition of chloroform: Methanol : Ethyl acetate (4:2:2 v/v/v) with 15 min time of saturation. The analytes were tracked down at 255 nm. The components were resolved with retention factor of losartan potassium (0.59 ± 1.98) and hydrochlorothiazide (0.83 ± 1.95). The method assured linearity in the concentration range of 600-1000 ng/spot for losartan potassium and 300-500 ng/spot for hydrochlorothiazide, respectively. Accuracy of method was assessed and found upto 100 % ± <2. The method assured high degree of precision. The robustness was confirmed by deliberately varying the parameters like composition of mobile phase, mobile phase volume, detection wavelength, chamber saturation time.