Two distinct classes of methicillin-resistant Staphylococcus aureus (MRSA) are spreading in hospitals (as hospital-acquired MRSA, HA-MRSA) and in the community (as community-acquired MRSA, CA-MRSA). Multilocus sequence type (ST) 239 MRSA, one of the most worldwide-disseminated lineages, has been noted as a representative HA-MRSA. Here, we isolated ST239 MRSA (spa type 3 [t037] and staphylococcal cassette chromosome mec [SCCmec] type III.1.1.1) and its novel variant with ST239/spa351 (t030)/SCCmecIII.1.1.4 (SCCmecIIIR) not only from hospitals but also from patients with urethritis in the community in Russia. The Russian variant (strain 16K) possessed a hybrid genome consisting of CC8 and CC30, similar to the ST239/spa3/SCCmecIII.1.1.1 HA-MRSA (TW20) genome, but with marked diversity. The 16K′ CC30 section had SCCmecIIIR carrying the dcs-carrying unit (which corresponded to the SCCmecIVc J3 joining region of ST30 CA-MRSA), lacked SCCmercury, and possessed a novel mobile element structure (MES16K) carrying the ccrC-carrying unit (with the recombinase gene ccrC1 allele 3) and drug resistance tranposons. The Russian variant included strains with a high ability to transfer its multiple drug resistance by conjugation; e.g., for strain 16K, the transfer frequency of a chloramphenicol resistance plasmid (p16K-1 with 2.9 kb in size) reached 1.4×10−2, followed by Tn554 conjugative transfer at 3.6×l0−4. The Russian variant, which has been increasing recently, included divergent strains with different plasmid patterns and pulsed field gel electrophoresis profiles. The data demonstrate the alternative nature of ST239 MRSA as CA-MRSA and also as a drug resistance disseminator, and its micro but dynamic evolution in Russia.

The numerical investigation of the flow regimes in the T-shaped microchannels with different width-to-height aspect ratios of mixing channel was carried out. In the first case, the mixing channel width was varied from 200 m to 1000 m while its height was constant; in the second case, the mixing channel height was varied from 100 m to 2000 m, while its width was constant. The Reynolds number was varied from 5 to 700. The dependences of fluids mixing efficiency and the pressure drop on the Reynolds number at different width-to-height aspect ratios of mixing channel were numerically established. The correlations to determine the critical Reynolds number, as well as the friction factor at different widths and heights of the mixing channel, were proposed. The mixing efficiency, reduced to the pressure drop and the volume unit was analyzed for the first time. The optimal range of parameters in terms of the working efficiency of the micromixer was determined.

The present investigation has been performed over a wide range of the dimensionless parameters characterizing the process of propagation of pressure perturbations in a gas-liquid mixture; these are the Reynolds number, and a dispersion parameter responsible for the relation between the values of dispersion and signal intensity. The values of the above parameters were changed mainly by varying the initial perturbation. The results obtained show a complete agreement between the Burgers-Korteweg-de Vries model and the real process of propagation of long-wave perturbations in a liquid with gas bubbles. In addition to signal propagation with the formation of monotonic and oscillatory shock waves, the propagation of signals in the form of solitary waves (solitons) and wave packets was observed experimentally. Data have been obtained on signal damping, energy dissipation and the influence of mixture viscosity on the signal evolution.

The combination of solifenacin in a double dosage along with tamsulosin can be recommended for elderly benign prostatic hyperplasia patients with overactive bladder symptoms.

No abstract