Background: Desensitizing solutions based on diamine silver fluoride with a high concentration of fluoride generate an occlusion of dentinal tubules. Objective: The aim was to evaluate the influence of time of application of diamine silver fluoride on the hydraulic conductance. Materials and Methods: The sample of this prospective experimental study included 60 healthy human third molars with indications of extraction in malocclusion. These were extracted from patients between 18 and 30 years of age. Four groups of 15 dentin disks were obtained: GC (no treatment), G1 (were actively treated with diamine silver fluoride for 15 s), G2 (30 s), G3 (60 s). Then, was measured hydraulic conductance of dentin. The rate of flow of the fluid through the dentin disks was measured by recording the initial position of a bubble of air inside a capillary in 20 min. Results: The hydraulic conductance obtained are: GC= 0,0433 (± 0.0210); G1= 0.0087 (± 0.0063); G2= 0.0082 (± 0.0050) and G3= 0.0061 (± 0.0040) expressed in µL/min*cm2. With the Wilcoxon signed rank test, it was established that there is a statistically significant difference in hydraulic conductance between the control group and the samples treated with desensitizing solution based on diamine silver fluoride/ potassium iodide. There was no statistically significant difference between the groups treated with different application times of the desensitizing solution. Conclusion: There was no difference in hydraulic conductance between disks with different application times of desensitizing solutions of diamine silver fluoride/potassium iodide.