Pinotage and Cabernet franc grape must were inoculated with Saccharomyces cerevisiae and Torulaspora delbrueckii yeasts. Differences in colour were observed between Pinotage (S. cerevisiae) and Pinotage (T. delbrueckii) wines, whereas differences in berry and herbaceous character were observed between Cabernet franc (S. cerevisiae) and Cabernet franc (T. delbrueckii) wines. Mouthfeel properties between treatments for both wines were not significantly different. Overall quality was slightly higher in wines inoculated with T. delbrueckii compared to wines inoculated with S. cerevisiae. Anthocyanins and flavanols measured in Pinotage wines made with T. delbrueckii were higher compared to Pinotage must inoculated with S. cerevisiae. Cabernet franc wines made with S. cerevisiae were higher in anthocyanin glycoside and flavanol concentrations compared to Cabernet franc wines made with T. delbrueckii. Insignificant differences in acetylated and coumarylated anthocyanins were evident between Cabernet franc (S. cerevisiae) and Cabernet franc (T. delbrueckii) wines. Principal component analysis showed that epigallocatechin gallate, epicatechin gallate, procyanidin B2, peonidin 3-O-glucoside, delphinidin 3-(6-acetyl) glucoside, petunidin 3-(6-acetyl) glucoside, malvidin 3-(6-acetyl) glucoside and malvidin 3-O-glucoside concentrations were highest in Pinotage wines inoculated with T. delbrueckii. Cabernet franc wines inoculated with S. cerevisiae yeasts were highest in malvidin 3-(6-p-coumaroyl) glucoside, petunidin 3-(6-p-coumaroyl) glucoside, petunidin 3-O-glucoside, epicatechin gallate and epigallocatechin gallate concentrations. Total anthocyanins were highest in Pinotage (S. cerevisiae) wines and Cabernet franc (T. delbrueckii) wines. Flavanols were highest in Pinotage (T. delbrueckii) and Cabernet franc (S. cerevisiae) wines. It is evident from the results that yeast species has an impact on the flavonoid concentrations within a grape variety.
Nine Torulaspora delbrueckii yeast strains, a commercial T. delbrueckii strain and a commercial Saccharomyces cerevisiae yeast strain were used in the production of small-scale Chenin blanc and Pinotage vinifications. The fermentations were carried out at 15°C and 24°C respectively. Four T. delbrueckii yeasts were used as single inoculants, while the remainder were inoculated sequentially. The commercial S. cerevisiae yeast strains were added at zero, 24 and 48 hours after the T. delbrueckii strain. The wines were evaluated chemically and sensorially and the data was analysed statistically. The results for the white wine vinification trial showed that two T. delbrueckii treatments could produce novel wines, either on their own or as a component of co-inoculated fermentations. These compared well with, and even exceeded, the quality of wine produced by the S. cerevisiae reference treatment regarding chemical composition and overall sensory quality. One T. delbrueckii strain showed its robustness by being re-isolated from the yeast lees at the end of fermentation. The red wine vinifications were less conclusive, and no distinctive T. delbrueckii "fingerprint" was observed in the chemical and sensory data, neither was a pattern observed regarding the different inoculation times.
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