Background and Objectives: The genetic background of the D^Va category phenotype has been described in two Caucasian individuals. We were interested in the RHD sequence of 7 Japanese D^Va individuals and their families. Materials and Methods: With SSP–PCR we tested exons 4, 5 and 7 of the RHD gene and used restriction enzymes for testing nucelotide associated with the D^Va phenotype. Results: A single RHD G667 C697 allele was present in 5 individuals with D^Va category phenotype, and in 2 individuals we found a D–CE–D hybrid gene (exon 5 had been replaced). The D^VaCe gene complex was found in all families. Conclusion: The changes of the RHD gene described in European D^Va individuals were also observed in Japanese families.

The changes of the RHD gene described in European D(Va) individuals were also observed in Japanese families.