The necessity of growing stevia in the Forest-Steppe and the Ukrainian Steppe has been theoretically substantiated and practically proved. Breeding methods and basic elements of stevia growing technology are developed on the basis of the plant biological characteristics, its adaptive potential and varieties analysis. Research results. The recommended technology has ensured the maximum implementation of the biological potential of stevia plants, which is manifested in more intensive growth and development of plants, significantly increased the yield of green mass and dry leaves compared with the control, respectively, by 6.2 and 1.1 t/ha. In order to obtain stevia dry leaves yield at the level of 3.5–4.0 t/ha it is recommended to sow the new generation varieties of domestic breeding: Halyna, Maryna, Kateryna; stevia propagation method was proposed (Patent No. 119472, 2017). Over the years of testing, the annual economic effect amounted to 585.1 thousand UAH. Conclusions. 1. The theoretical basis of the recommended stevia cultivation technology is the determination of the patterns of leaf mass formation, photosynthetic potential and the net productivity of photosynthesis depending on the varietal composition of the elements of growing technology and weather conditions of the growing season. 2. To get a yield of stevia dry leaves at the level of 3.5–4.0 t/ha it is recommended: - to grow stevia in the regions where the annual amount of precipitation year is 460–560 mm, including 322-460 mm for the vegetation period, the moisture content in 100 cm soil layer – 150–180 mm, the amount of temperature during the period of active vegetation – 2450–2800 °C, STC – 0.8-1.3; - to sow varieties of domestic breeding: Berehynya, Slavutych, Halyna, Maryna, Kateryna. 3. Growing stevia by seedlings method should be carried out on May 20–30 in a 70x16 cm pattern. Key words: stevia, introduction, cultivation technology, varieties, reproduction methods, fertilization, seeds stimulation, yield.
The article presents the results of long-term research on the growth, development and storage of stevia collection. The optimum composition of the culture medium and the temperature when cultured in vitro determined to be low above-zero temperatures.
Purpose. It is theoretically possible to substantiate and practically determine the degree of growth and development of stevia plants and their productivity depending on the use of the Avatar-2 microfertilizer. Methods. Laboratory, field, analytical, statistical. Results. During the years of research, the highest productivity of stevia agrophytocenoses was obtained in the variant with soaking (pre-sowing) of seeds in microfertilizer Avatar-2 + twice foliar fertilization. Plant density before harvest was by 10.5 thousand/ha, yield of green mass by 3.3 t/ha and including leaves by 2.5 t/ha, dry matter yield by 0.7% (including leaves by 0.3%) higher than in the control. Conclusions. The main effect of soaking seeds in the trace element formulation Avatar-2 (registered in Ukraine in 2006) in the technology of growing stevia is to increase the efficiency and uniformity of seed germination in both laboratory and field conditions, which promotes more intensive growth and development of plants during the vegetation, and when applied in the end of vegetation, to better preserve them and increase the yield of green and dry matter.
Через 5 діб культивування новоутворені пагони відокремлюються, реєструються з визначенням вегетативного покоління колхіцинованого втечі. Друге вегетативне покоління пагонів, в кількості не менше 3 штук, після двох тижнів культивування використовували для визначення плоїдності і відбору тетраплоїдов. Для синхронізації ділення клітин меристеми колби ставили в холодильник на 12 годин при температурі +4 °С , а потім на 2,5-3 години на світло для отримання 3000-4000 лк. В таких умовах отримують до 37% плоїдності тетраплоїдних форм стевії з високою продуктивністю і стійких до грибкових захворювань. Ключові слова: поліплоїдизація, колхіцин, стевія, культура in vitro, тетраплоїди Актуальність. Упродовж тисячоліть індіанці племені гуарані на території сучасної Бразйлії та Парагваю використовували в їжу деякі види стевії, в особливості Stevia rebaudiana, яку вони називали ka'ahe'e («солодка трава») в якості підсолоджувача до мате і інших медичних чаїв, для лікування різноманітних хвороб [1]. За деякими припущеннями, свою назву стевія отримала завдяки російському ботаніку шведського походження X. X. Стевену (1781-1863) [4]. Вперше вона була досліджена іспанським лікарем і ботаніком Стевусом (лат. Petrus Jacobus Stevus/ ісп. Pedro Jaime Esteve), за прізвищем якого отримала латинську назву. В подальшому розпочалась праця з виготовлення екстракту,
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