Objectives: Health is defined as a state of complete physical, mental, and social well-being. Materials and Methods: Humans have settled in both urban and rural areas, which might have influenced people and their physiology in different ways. Results: Cardiovascular fitness as a marker of proper functioning of the whole body is associated with many health-related outcomes, with poor fitness leading to development of cardiovascular diseases (CVD). Conclusion: This study aimed to perform a comparative study of the physiological parameters’ indicative of cardiovascular fitness in urban and rural adolescent girls.