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The aim of the research was to study the possibility of using immunohistochemical markers for the diagnosis of intravital stangulation. Materials and methods: Fragments of the neck skin from the strnaglulation zone were selected as objects of research. The main study group included 20 deaths due to hanging (10 men and 10 women). For the control group, 10 cases of acute coronary death (5 men and 5 women) were used. Using a complex of IHC markers, the labeling of the epidermis and epithelial structures, the features of cellular immune responses, the manifestation of oxidative steress were studied. Results: Peculiarities of morphological manifestations of strangulation furrow in mechanical asphyxia were studied. It is established that the key link of its morphogenesis is impaired vascular wall permeability with loss of type IV collagen in the basement membranes of epithelium and skin vessels, migration into tissues of activated CD15+ granulocytes, CD68+ macrophages and CD117+ labrocytes, endothelial nitric oxide synthase (eNOS), inducible nitric oxide synthase (iNOS), fibrinogen protein and transforming growth factor β1. Conclusion: Such changes in the tinctorial properties of skin and subcutaneous adipose tissue can be recorded by immunohistochemical (IHC) and serve as an important diagnostic criterion for the viability of the formation of the strangulation furrow.

Purpose – to analyze the state of the level of research and macroscopic diagnosis by forensic experts of corpses in cases of strangulation asphyxia by sectional methods, detection of specific and general asphyxial signs.Material and methods. As objects of research, the maintenance of «Conclusions of the expert» was studied, first of all, for their compliance with the «Rules of carrying out forensic medical examination (research) of corpses in the bureau of forensic medical examination» on the following indicators: 1) quality and completeness of description of external damages, dimensional parameters; 2) completeness of internal research; 3) description and reflection of species and general asphyxiation features; 4) referral to laboratory research methods; 5) use of additional sectional techniques; 6) completeness and correctness of summarizing.Results and conclusions. In accordance with the modern requirements of evidence-based medicine, when conducting forensic expert examinations of corpses with strangulation asphyxia, it is not enough to rely only on macroscopic signs when conducting forensic medical expert examinations of corpses with strangulation asphyxia. Our analysis of archival data from the "Expert Conclusions" suggests that most experts treat their responsibilities irresponsibly, do not attach importance and difference between such concepts as species characteristics of strangulation and general asphyxiation. In compiling the results, experts rely only on the presence of a strangulation furrow and general asphyxiation signs. In the “Conclusions of experts” we analyzed, some species traits were unjustifiably classified as general asphyxiation manifestations, and some were not paid attention to or were not studied at all.