Developing new barrier membranes with improved biomechanical characteristics has acquired much interest owing to their crucial role in the field of periodontal tissue regeneration. In this regard, we enriched the electrospun polycaprolactone (PCL)/gelatin (Gel) membranes by adding bioglass (BG) or Cu-doped bioglass (CuBG) and examined their cellular adhesion and proliferation potential in the presence of alveolar bone marrow-derived mesenchymal stem cells (aBMSCs). The membranes were fabricated and characterized using mechanical strength, SEM, FTIR, EDX, and ICP assay. Besides, aBMSCs were isolated, characterized, and seeded with a density of 35,000 cells in each experimental group. Next, the cellular morphology, cell adhesion capacity, proliferation rate, and membrane antibacterial activity were assessed. The results displayed a significant improvement in the wettability, pore size, and Young’s modulus of the PCL membrane following the incorporation of gelatin and CuBG particles. Moreover, all scaffolds exhibited reasonable biocompatibility and bioactivity in physiological conditions. Although the PCL/Gel/CuBG membrane revealed the lowest primary cell attachment, cells were grown properly and reached the confluent state after seven days. In conclusion, we found a reasonable level of attachment and proliferation of aBMSCs on all modified membranes. Meanwhile, a trace amount of Cu provided superiority for PCL/Gel/CuBG in periodontal tissue regeneration.

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Background and aims. Changes in the balance of sexual hormones during pregnancy decrease gingival crevicular fluid levels of interleukin-6 and the resistance of gingival tissue against inflammations. Hormonal contraceptives are agents that are based on the effects of gestational hormones and simulate a state of pregnancy; therefore, they prevent ovulation. This study evaluates the effect of these drugs on periodontal tissues and levels of IL-6 in gingival crevicular fluid. Materials and methods. Twenty-five patients who had not used oral contraceptives (control) and 35 patients using oral contraceptives (case) were examined clinically and their medical history, dosage and duration of oral contraceptives use were recorded. Periodontal indices such as bleeding on probing, plaque index, probing pocket depth, clinical attachment loss and levels of IL-6 in gingival crevicular fluid were measured. Student’s t-test and Mann-Whitney test were used to analyze data. Results. Mann-Whitney test showed a statistically significant difference in the mean of bleeding on probing in the case and control groups (P<0.05). Student’s t-test showed a statistically significant difference in the mean of IL-6 levels (P<0.05), probing pocket depth (P<0.05) and clinical attachment loss between the case and control groups (P<0.05) but no statistically significant differences were found between the plaque index of the case and control groups (P>0.05). Conclusion. It seems that use of oral contraceptives may affect the periodontal health status of patients, leading to more gingival inflammation. Therefore, patients must have a strict oral hygiene care.