In this paper, a different method for de-noising of ECG signals using wavelets is presented. In this strategy, we will try to design the best wavelet for de-nosing. Genetic algorithm tests wide range of quadrature filter banks and the best of them will be chosen that minimize the Signal-to-Noise Ratio (SNR). Furthermore, the wavelet function and scaling function related to these filters are reported as the best wavelet for de-noising. Simulation results for de-noising of a noisy ECG signal show that using obtained wavelet by proposed method improves the SNR of about 2.5 dB.