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Background & Objective: Location of sensors is an important information in wireless sensor networks for monitoring, tracking and surveillance applications. The accurate and quick estimation of the location of sensor nodes plays an important role. Localization refers to creating location awareness for as many sensor nodes as possible. Multi-stage localization of sensor nodes using bio-inspired, heuristic algorithms is the central theme of this paper. Methodology: Biologically inspired heuristic algorithms offer the advantages of simplicity, resourceefficiency and speed. Four such algorithms have been evaluated in this paper for distributed localization of sensor nodes. Two evolutionary computation-based algorithms, namely cultural algorithm and the genetic algorithm, have been presented to optimize the localization process for minimizing the localization error. The results of these algorithms have been compared with those of swarm intelligence- based optimization algorithms, namely the firefly algorithm and the bee algorithm. Simulation results and analysis of stage-wise localization in terms of number of localized nodes, computing time and accuracy have been presented. The tradeoff between localization accuracy and speed has been investigated. Results: The comparative analysis shows that the firefly algorithm performs the localization in the most accurate manner but takes longest convergence time. Conclusion: Further, the cultural algorithm performs the localization in a very quick time; but, results in high localization error.