Following an industrial process to protect manufactured glass windows against atmospheric corrosion during their storage and transport, this research is focused on the possibility that a surface deposit of a small amount of zinc salts may efficiently reduce the atmospheric alteration kinetics of ancient glasses (soda, potash, and mixed alkali silicates). To this end, the chemical action of zinc salts was investigated by means of aging experiments (with temperature and relative humidity (RH) control) on three glass models, of which compositions are representative of the cultural heritage. When the treatment was performed on pristine glass (about 1 μg/cm2 of deposited Zn2+ ions), treated glass plates developed a significantly thinner hydrated layer than the untreated ones at 80°C or at 40°C (85 RH%). The formation of alkali and Ca‐carbonates salts on the surface was also considerably reduced. The state of the glass surface undergoing the Zn treatment and the temperature of the treatment seem critical regarding the efficacy of the protection.

Organophosphorus nerve agent (OPNA) adducts formed with human butyrylcholinesterase (HuBuChE) can be used as biomarker of OPNA exposure. Indeed, intoxication by OPNAs can be confirmed by the LC/MS analysis of a specific HuBuChE nonapeptide on which OPNAs covalently bind. A fast, selective, and highly sensitive online method was developed to detect sarin and soman adducts in plasma, including immunoextraction by anti-HuBuChE antibodies, pepsin digestion on immobilized enzyme reactors (IMER), and microLC/MS analysis of the OPNA adducts. The potential of three different monoclonal antibodies, covalently grafted on sepharose, was compared for the extraction of HuBuChE. The online method developed with the most promising antibodies allowed the extraction of up to 100% of HuBuChE contained in plasma and the digestion of 45% of it in less than 40 min. Moreover, OPNA-HuBuChE adducts, aged OPNA adducts, and unadducted HuBuChE could be detected (with S/N > 2000), even in plasma spiked with a low concentration of OPNA (10 ng mL). Finally, the potential of this method was compared to approaches involving other affinity sorbents, already described for HuBuChE extraction. Graphical abstract Online coupling of immunoextraction, digestion, and microliquid chromatography-tandem mass spectrometry for the analysis of organophosphorous nerve agent adducts formed with human butyrylcholinesterase.