Mosaicism is a major issue for SCA cfDNA testing, and prenatal confirmation, preferentially with amniocentesis if there are no ultrasound anomalies, remains important in counseling. As PPV varies on the basis of the presence of an ultrasound anomaly, skilled evaluation is critical. © 2017 John Wiley & Sons, Ltd.

uncultured amniocytes for the targeted chromosome combined with a standard karyotype analysis can also be taken into consideration. 16 In conclusion, on the basis of our cytogenetic experience, the new indication for invasive prenatal diagnosis 'increased risk after NIPS on cell-free DNA circulating in maternal blood' requires new specific criteria for confirmatory cytogenetic analysis on amniocytes, and the appropriate communication by clinicians of the specific risk allows the application of appropriate laboratory procedures. A discussion of the possible supplementation of the current cytogenetic best practice guidelines is advisable.

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Objectives: To assess the incidence and outcomes of genetic polymorphism in high-risk obstetric and subfertile populations. Methods: From January 2017 to February 2019, 78 patients were detected to have genetic polymorphisms. 35% are high-risk obstetric women who were followed up until their outcomes and 65% are from the subfertile group who are currently being followed up with assisted reproductive techniques. Results: Out of the pregnancies 89% delivered, 4% had miscarriages and 7% are being followed up with high-risk fetomaternal surveillance. Polymorphisms identified in only the subfertile population include Yqh+, Iqh+, 9qh-, Pstk+, 47XXY[1]/46XY[19] and 45XX, t(13, 14)(q12.1, q11.2).The incidence of detected aberrations in this group are Yqh+(4%), Yqh-(12%), Iqh+(2%), 9qh+(32%), 9qh-(4%), Pstk+(8%), 13pstk+(46%), 14pstk+(40%), 15pstk+ (50%), 21pstk+(58%), 22pstk+(42%), 14PSQ(2%), 47XXY[1]/ 46XY[19](2%) and 45XX, t(13, 14)(q12.1, q11.2)(2%). The incidence of genetic abnormalities in the subfertile population consists of Yqh+(4%), Yqh-(10%), 9qh+(24%), 9qh-(4%), Pstk+(8%), 13pstk+(26%), 14pstk+(24%), 15pstk+(34%), 21pstk+(36%), 22pstk+(26%), 47XXY(1)/46XY(19)(2%) and 45XX, t(13, 14)(q12.1, q11.2)(2%). The commonest abnormalities identified in the high risk obstetric group include 9qh+, 13pstk+, 14pstk+, 15pstk+, 21pstk+ and 22pstk+. The incidence of genetic abnormalities consists of Yqh-(4%), 9qh+(15%), 13pstk+(48%), 14pstk+(30%), 15pstk+(30%), 21pstk+(41%), 22pstk+(26%), and 14PSQ(4%). Genetic abnormalities associated with delivery of a low birth weight neonate are 9qh+, 13pstk+, 14pstk+, 15pstk+, 21pstk+ and 22pstk+. Incidence of recurrent miscarriages in this high risk population had underlying genetic abnormalities of Yqh+, 1qh+, 9qh+, pstk+, 13pstk+, 14pstk+, 15pstk+, 21pstk+, 22pstk+ and 14PSQ. Conclusions: Genetic polymorphism is responsible for bad pregnant and fertility outcome in high risk population. Further studies are needed to identify genetic variations and their contribution to bad outcomes. OP05.07Outcomes following the detection of fetal edema in early pregnancy prior to non-invasive prenatal testing