An improved concept of the best analogs method is used to reconstruct the climate of the last glacial maximum from pollen data in Europe. In order to deal with the lack of perfect analogs of fossil assemblages and therefore to obtain a more accurate climate reconstruction, we used a combination of pollen types grouped according to plant phenology and present climate constraints rather than pollen percentages for each individual taxon. The distribution of pollen taxa into plant functional types (PFTs) is aimed to reflect the vegetation in terms of biomes which have a wider distribution than a species. The climatic variables are then calibrated on these PFTs using an artificial neural network technique. The use of PFTs allowed us to deal with situations where pollen assemblages have only partial modern analogs. The method is applied to the glacial steppic vegetation in Europe, using 15 pollen records. North of the Pyrenees–Alps line, the reconstructed temperatures were lower than today: −30 ± 10°C for the temperature of the coldest month ( Tc) and −12 ± 3°C for the annual mean ( Tann). South of that line, Tc and Tann anomalies were respectively, −15 ± 5°C and −10 ± 5°C. The available moisture index and annual precipitation were also lower than present: −60 ± 20% north of Mediterranean Sea, (−800 ± 100 mm for precipitation). In Italy and Greece, the available moisture was 20% lower, with a precipitation anomaly of ca. −600 ± 200 mm. Southward, the moisture index was close to that at present (±20%), and precipitation was lower (−300 ± 300 mm).

Pollen data from 18,000 14 C yr bp were compiled in order to reconstruct biome distributions at the last glacial maximum in southern Europe and Africa. Biome reconstructions were made using the objective biomization method applied to pollen counts using a complete list of dryland taxa wherever possible. Consistent and major differences from present-day biomes are shown.Forest and xerophytic woods/scrub were replaced by steppe, both in the Mediterranean region and in southern Africa, except in south-western Cape Province where fynbos (xerophytic scrub) persisted.Sites in the tropical highlands, characterized today by evergreen forest, were dominated by steppe and/or xerophytic vegetation (cf. today's Ericaceous belt and Afroalpine grassland) at the last glacial maximum.Available data from the tropical lowlands are sparse but suggest that the modern tropical rain forest was largely replaced by tropical seasonal forest while the modern seasonal or dry forests were encroached on by savanna or steppe. Montane forest elements descended to lower elevations than today.

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Vascular endothelial growth factor (VEGF ), an endothelial cell mitogen, is a potent angiogenic factor produced by several cell types. Whether human neutrophils are potential producers of VEGF has not yet been described. The present work shows that phorbol-12-myristate 13-acetate (PMA), fMet-Leu-Phe, and tumor necrosis factor-α (TNF-α) triggered a time-dependent secretion of VEGF by human neutrophils. Cells incubated with 50 ng/mL of PMA released significant amounts of VEGF after 15 minutes. Because the extracellular content of VEGF in human neutrophils supernatants remained constant over a period of 2 to 24 hours and because PMA is a potent inducer of human neutrophil degranulation, the PMA-induced secretion of VEGF may be due to a pre-existing intracellular pool of this molecule. This hypothesis was reinforced by the absence of cycloheximide effect on the PMA-induced secretion of VEGF. The existence of an intracellular pool of VEGF was confirmed by measuring the intracellular content of VEGF in resting neutrophils. A dosedependent inhibition of PMA-induced VEGF secretion was observed when the cells were incubated in the presence of pentoxifylline, a methylxanthine known to inhibit neutrophil degranulation. To confirm the implication of neutrophil degranulation in VEGF release, the effects of two inducers of physiologic degranulation, fMet-Leu-Phe and TNF-α, were determined. Both agonists induced a release of VEGF in the absence of cytochalasin B, confirming the involvement of neutrophil degranulation and suggesting the intracellular localization of VEGF in the specific granule fraction. In addition, the kinetics of fMet-Leu-Phe– and TNF-α–induced secretion of lactoferrin were similar to those of VEGF release induced by these two both agonists. The subcellular fractionation of human neutrophils showed a granule-specific distribution of the intracellular pool of VEGF in resting neutrophils. The finding that human neutrophils contain an intracellular pool of VEGF, secreted in the extracellular space under PMA-, fMet-Leu-Phe–, and TNF-α–induced degranulation, suggests a role for human neutrophils as cellular effectors of physiologic as well as pathologic angiogenesis.