A new procedure for the determination of uranium by packed-column supercritical fluid chromatography is proposed. A nonfluorinated chelating agent selective for copper and uranium, the 2,6-diacetylpyridine bis(benzoylhydrazone), has been chosen. We have studied its chromatographic properties on different stationary phases and the influence of the methanol content in the carbon dioxide mobile phase. The separation of the metal compounds was conducted with and without solvent injection. A calibration curve was obtained for uranium in the range of 52-323 ng injected. The accuracy of the method is 0.5%, the repeatability 4%. The same studies were performed with a new compound, diacetyl-2,6 pyridine bis(4-tert-butyl benzoylhydrazone). An increase in retention and efficiency was then observed.