Hepatitis C is an emerging infection in India whose long term implications will be felt in the decades to come. In the present study the seroprevalence of HCV infection among 85 healthcare workers was determined. Blood samples were collected from healthcare workers which included doctors, nurses, lab technicians and lab attenders and the sera was tested for anti-HCV antibodies using third generation immunoassay kit. In our study the prevalence of HCV was 1.17%. It is imperative that health care workers be sensitized about universal precautions and safe disposal of needles and other contaminated materials to decrease the risk of infection through continuous medical education programmes. Our study supports that the screening of HCV among healthcare workers is mandatory.