Highlights d Atrioventricular valvulogenesis in zebrafish is regulated by Notch and Klf2 pathways d Notch-mediated lateral inhibition singles out Delta-like-4positive endocardial cells d These cells ingress into the cardiac jelly in response to Erk5-Klf2-Wnt9a signaling d Frizzled9b makes presumptive ingressing endocardial cells competent to respond to Wnt9a
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