Tanticharoenia aidae sp. nov. was introduced for two strains, designated as VTH-Ai06 T and VTH-Ai07, which were isolated from the stems of sugar cane collected in Vietnam. In a phylogenetic tree based on 16S rRNA gene sequences derived from the neighbor-joining method, the two strains formed a cluster along with the type strain of Tanticharoenia sakaeratensis with a bootstrap value of 64 %. The pair-wise 16S rRNA gene sequence similarity was 98.3 % between strain VTH-Ai06 T and the type strain of T. sakaeratensis. The range of DNA G+C contents was from 65.2 to 65.4 mol%. In DNA-DNA hybridization, the two strains showed low similarities to T. sakaeratensis BCC 15772 T . Cellular fatty acids were of C 18:1 ω7c as a major. A major isoprenoid quinone was Q-10. Phenotypically, the two strains differed from the type strain of T. sakaeratensis in no production of dihydroxyacetone from glycerol. The type strain is VTH-Ai06 T (= VTCC 910001 T =BCC 67839 T = NBRC 110637 T ), whose DNA G+C content was 65.4 mol%.
Two Nguyenibacter vanlangensis strains TN01LGIT and VTH-AC01 isolated by an enrichment approach using nitrogen free LGI medium from Asian rice isolation sources are expected as an additional PGPB within the Acetobacteraceae family. In this study, nitrogen fixing capability of the two N. vanlangensis strains was confirmed through nitrogenase activity’s detection by acetylene reduction analysis...
Sixty-six acetic acid bacteria (AAB) were isolated from fourty-five flowers and fruits collected in Hochiminh City, Vietnam. Of the sixty-six, thirty-one isolates were selected as dihydroxyacetone (DHA)-producing AAB based on the reaction with Fehling’s solution and grouped into three groups by routine identification with phenotypic features. Group I composed of fourteen isolates and was assigned to the genus Acetobacter, Group II composed of thirteen isolates and was assigned to the genus Gluconobacter and Group III was the remaining four isolates and was assigned to the genus Gluconacetobacter. Ten isolates among the thirteen isolates of Group II gave a larger amount of DHA (22.2–26.0 mg/mL) than Gluconobacter oxydans NBRC 14819T (19.8 mg/mL), promising for the potential use in producing DHA. In phylogenetic analysis based on 16S rRNA gene sequences, six isolates of the ten potential DHA producers were suggested to be candidates for new taxa in the genus Gluconobacter.
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