This study aimed to measure the safety culture of the patient from the perspective of the multiprofessional team, working in a hospital. This was a cross-sectional quantitative study carried out at a hospital institution. The data collection was performed in October 2017, and the Safety Attitudes Questionnaire was used. Data analysis was performed using descriptive and analytical statistics. The prevalence of the female gender, an age range between 31 and 40 years, and full secondary schooling were identified. Among the domains, satisfaction with work was the only one that presented a positive mean (88.18±14.63). When comparing the nursing and support team groups there was a significant difference in the domains of job satisfaction, perception of stress, unit and hospital management. In addition, it was identified that work characteristics such as overtime, place of work and time in the institution are also variables that interfered with the evaluation of the SAQ. The safety culture was assessed as negative by the institution's professionals. This place requires an urgent intervention, a multiprofessional work, to recognize the need for changes in organizational culture, with the implementation of actions and strategies that benefit the quality of care as a whole, in favor of patient, professional and the Hospital Institutional safety.