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The common bean (Phaseolus vulgaris) has recently gained popularity as a dietary supplement substitute for enhancing human health. Humans need nutrition on a basic level; however, many people still struggle to meet their Zn and Fe requirements. In plants the uptake, transport, and accumulation of microelements (Zn and Fe) are significantly regulated by the ZIP transporter. Here, we present information on the ZIP gene of metal transporters in Phaseolus vulgaris. ZIP gene was identified through PCR which resulted of 133bp and sequence of ZIP gene having 235bp, was submitted to NCBI with accession no. MH845642 after BLAST analysis. The resulted sequence was further analysed In-silico viz. BioEdit, Mega5 and a phylogenetic tree was constructed. The expression level of ZIP gene was measured by Q-PCR using cox gene as an internal control. Different expression levels were obtained for 3, 6, and 12hrs time periods of exposure to zinc treatment at different concentrations 100 mM, 150 mM and 200 mM respectively. The relative expression of treated and untreated (control) of P. vulgaris cotyledons was calculated using BioRad IQ-5 software. Overall ZIP genes expression was highest at 200 mM for 12 hr (3.5fold) followed by zinc treatment at 200 mM for 6 hr (2.3fold) and lowest at 150 mM for 3hr (0.5fold). This study systematically analysed the gene structure, expression profiles, regulatory network, and the biological function of the ZIP family in Phaseolus vulgaris L., providing better understanding of the regulatory roles of ZIP genes and contributing to improve nutrient quality of crops.

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