The article proposes the method of setting the time moments of biomedical signal disruption, in particular, the electroencephalographic (for establishing the time moments of the appearance of the processes of the speech process) and the electrical probe signals (for setting the time moments of the beginning and the end of the polymerization process) based on the application of the sliding window. The parameters of the window (its width and the value of the shift step) are substantiated. The results of elaboration of EEG signals within the sliding window are analyzed and it is established that the maximum accuracy of setting the time moments of the appearance of the disorder will be achieved in the case of the maximum overlapping of the previous and next windows, that is, with a minimum displacement value (in the case of discrete sequences, the minimum displacement value will be equal to the sampling period).