The performance of the Proton Exchange Membrane Fuel Cell (PEMFC) depends on various design and operating parameters. The present work is aimed to optimize the performance of 25cm 2 PEMFC, with respect to one design parameter (landing to channel width ratio-L: C) of serpentine flow field and two operating parameters (cell temperature & back pressure). Here, Taguchi technique and analysis of variance methodology have been used to obtain the optimum combination for the above three parameters. The experiments have been carried out with respect to Taguchi's orthogonal array of L9 (33), for parametric analysis of PEMFC performance. Three factors and three levels, such as various landing width to channel width ratio (1:1, 1:2 and 2:2), cell temperature (40 o C, 50 o C and 60 o C) and back pressure (0, 0.5 and 1 bar) have been taken for optimization studies. Based on the experimental study conducted on serpentine flow field, the maximum power density of 0.263 W/cm 2 has obtained at 1 bar back pressure, 60°C cell temperature and L:C-1:2. Among all the design and operating parameters, the major parameter that contributes to the performance of PEMFC is back pressure with 69.8 %.