During our investigation on bioactive antifungal pigments the strain Chrysosporium queenslandicum IFM 51121 was found to produce a series of darkish to reddish pigments which were identified as naphthaquinone-type altersolanols A, B, C1-3). Moreover, a new antifungal compound (queenslandon)4) was isolated as a new representative of the zearalenone family of mycotoxin. In addition to the above metabolites C. queenslandicum IFM 51121 co-produced new antibacterial compounds related to members of the dihydronaphthaquinone group5-7) which were given the name chrysoqueen (1) and chrysolandol (2).In this paper we report fermentation, isolation, physicochemical properties and the structure elucidation of 1 and 2. the residue was dissolved in a small amount of ethyl acetate and further purification and separation was achieved by preparative thin-layer chromatography on silica gel plates (Merck silica gel 60 F254) using mixtures of v/v) and (80:20; v/v). The compounds were disclosed on the TLC plates due to their yellowish fluorescence in UV254 light.

In the course of our screening program for biologically active compounds from pathogenic microorganisms, an antibacterial and antifungal naphthoquinone group antibiotic complex was isolated from the culture broth of a strain Chrysosporium queenslandicum IFM 51121. The compounds of the complex were identified as altersolanol A, B and C1-3). Besides the antibiotics, the strain C. queenslandicum IFM 51121 produces also a new antifungal compound related to zearalenone family of mycotoxin4), designated queenslandon (1).In this paper we report the production, isolation and structure elucidation of the mycotoxin queenslandon ( Fig. 1), through the use of one and two dimensional NMR techniques and mass-spectrometry.C. queenslandicum IFM 51121 strain was isolated from a soil sample collected in Egypt, and deposited in the collection of Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Japan.The mature slant culture of C. queenslandicum IFM 51121 was inoculated into a 50ml Erlenmeyer shake flask containing 25ml of a seed medium (2% glucose supplemented BHI medium). The inoculated flask was

Antibiotics Antibiotics U 1200Isolation and Structure Elucidation of Two New Antibacterial Compounds Produced by Chrysosporium queenslandicum. -The fermentation, isolation, physicochemical properties and structure elucidation of two new antibacterial compounds named as chrysoqueen (I) and chrysolandol (II) are described. (I) and (II) show activity against Gram-positive bacteria, e.g. Bacillus subtilis PCI. -(IVANOVA, V. B.; HOSHINO, Y.; YAZAWA, K.; ANDO, A.; MIKAMI*, Y.; ZAKI, S. M.; GRAEFE, U.; J. Antibiot. 55 (2002) 10, 914-918; Res. Cent. Pathog. Fungi Microb. Toxicoses, Chiba Univ., Chuo, Chiba 260, Japan; Eng.) -H. Haber 13-197

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