A yellow Gram-stain-positive, non-motile, non-endospore -forming, spherical endophytic actinobacterium, designated strain AE-6T, was isolated from the inner fleshy leaf tissues of Aloe barbadensis (Aloe vera) collected from Pune, Maharashtra, India. Strain AE-6T grew at high salt concentrations [10 % (w/v) NaCl], temperatures of 15–41 °C and a pH range of 5–12. It showed highest (99.7 %) 16S rRNA gene sequence similarity with Micrococcus yunnanensis YIM 65004T followed by Micrococcus luteus NCTC 2665T (99.6 %) and Micrococcus endophyticus YIM 56238T (99.0 %). Ribosomal protein profiling by MALDI-TOF/MS also showed it was most closely related to M. yunnanensis YIM 65004T and M. luteus NCTC 2665T. Like other members of the genus Micrococcus , strain AE-6T had a high content of branched chain fatty acids (iso-C15 : 0 and anteiso-C15 : 0). MK-8(H2) and MK-8 were the predominant isoprenoid quinones. Cell wall analysis showed an ‘A2 l-Lys-peptide subunit’ type of peptidoglycan and ribose to be the major cell wall sugar. The DNA G+C content was 70 mol%. Results of DNA–DNA hybridization of AE-6T with its closest relatives from the genus Micrococcus produced a value of less than 70%. Based on the results of this study, strain AE-6T could be clearly differentiated from other members of the genus Micrococcus . We propose that it represents a novel species of the genus Micrococcus and suggest the name Micrococcus aloeverae sp. nov., with strain AE-6T ( = MCC 2184T = DSM 27472T) as the type strain of the species.

). The genomic DNA G+C content was 37.4 mol% and the strain showed 37.7 % DNA-DNA relatedness to D. robiginosus DSM 25058 T . The major fatty acids were anteiso-C 15 : 0 , C 16 : 0 , iso-C 15 : 0 and iso-C 16 : 0 and MK-6 was the predominant quinone. The polar lipid profile of strain SD111T consisted of unidentified phospholipids (PL1 and PL2), phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG). The cell wall contained mesodiaminopimelic acid and the peptidoglycan was of A1c type. Glucose and ribose were detected as major cell-wall sugars. Results from polyphasic studies indicated that SD111 T represents a novel species of the genus Domibacillus for which the name Domibacillus indicus sp. nov. is proposed. The type strain is SD111 T (5MCC 2255 T 5DSM 28032 T ).The genus Domibacillus was described by Seiler et al. (2013) to incorporate a strain of a bacterium that formed red-pigmented colonies isolated from a pharmaceutical clean room in eastern Germany based on its morphological, chemotaxonomic and phylogenetic differences from closely related members of the genera Bacillus, Jeotgalibacillus and Planococcus. Taxonomically Domibacillus belongs to the phylum Firmicutes, class Bacilli, order Bacillales and family Bacillaceae (Parte, 2014). At the time of writing only a single species (Domibacillus robiginosus) with a validly published name is included in the genus Domibacillus. Members of the genus Domibacillus are Gram-stain-positive, spore-forming, oxidative, motile and strictly aerobic rods. The presence of MK-6 as the dominant quinone is one of the characteristic features of the genus. The cell-wall peptidoglycan is of A1c type, with meso-diaminopimelic acid, and glucose and ribose are dominant cell-wall sugars. Based on differences in phylogenetic, chemotaxonomic and phenetic characteristics between a novel strain, SD111 T , and D. robiginosus DSM 25058 T , in current study we propose that strain SD111T represents a novel species of the genus Domibacillus. Strain SD111T was isolated from a sediment sample collected from Lakshadweep, India, using seawater agar (HiMedia) after 72 h of incubation at 30 u C. Purity of the culture was checked by restreaking several times on the same medium, microscopic observation and 16S rRNA gene sequencing. Glycerol (20 % w/v) stocks of pure culture were preserved in liquid nitrogen at the Microbial Culture Collection (MCC), National Centre for Cell Science (Pune, India) for further study. For SSU rRNA gene sequencing, genomic DNA was extracted using a standard phenol/ chloroform method (Marmur 1961) and the 16S rRNA gene sequence was amplified using the 27F (AGAGTTTGATC-MTGGCTCAG) and 1492R (GGTTACCTTGTTACGAC-TT) eubacterial primers (Lane, 1991). The PCR product was purified using a Rapid Tip kit from Diffinity Genomics and DNA sequencing was carried out using an ABI PRISM Big Dye Terminator v3.1 Cycle Sequencing kit on a 3730xl Genetic Analyzer (Applied Biosystems). The sequence data were edited with the ChromasPro software as discussed by Prakash et al. (2014). A cont...

A Gram-stain-positive, rod-shaped, non-motile bacterium, strain PRD07, was isolated from Godavari river, India during the world's largest spiritual and religious mass bathing event 'Kumbh Mela'. Molecular analysis using 16S rRNA gene sequencing and phylogenetic analysis reveals the distinct phylogenetic positioning of strain PRD07 within the genus Corynebacterium. The strain demonstrated highest sequence similarity to Corynebacterium imitans DSM 44264 (97.9 %), Corynebacterium appendicis DSM 44531 (97.1 %) and <96.7 % with all other members of the genus Corynebacterium. The G+C content of PRD07 was 68.5 mol% (Tm) and the DNA-DNA hybridization depicts 61.09 % genomic relatedness with C. imitans DSM 44264. Chemotaxonomic assessment of strain PRD07 suggested presence of C16 : 0 (31.6 %), C18 : 0 (3.5 %) and C18 : 1ω9c (58.6 %) as the major cellular fatty acids. The major polar lipids of strain PRD07 were phosphatidylglycerol, diphosphatidylglycerol and glycophospholipid. Differentiating molecular, phylogenetic and chemotaxonomic characteristics of strain PRD07 with its closest relatives necessitated the description of strain PRD07 as a novel species of genus Corynebacterium for which the name Corynebacteriumgodavarianum sp. nov., has been proposed. The type strain is PRD07 (=MCC 3388=KCTC 39803=LMG 29598).

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