The content of biologically active substances and the level of antioxidant activity in the aerial organs of different sexual forms of Sibiraea altaiensis (Laxm.) Schneid. during the growing season were investigated for the first time. It was found that the maximum content of biologically active compounds varies depending on the stage of development, plant organ and also gender. The amount of flavonols (5.69%), tannins (30.17%) is higher in the leaves of male plants during the budding of plants, catechins ‒ in inflorescences (0.61%) of female plants, pectins ‒ in male buds (1.54%), protopectins ‒ in the leaves of males (8.99%) in the budding phase and in the leaves of females (9.62%) in the fruiting phase of plants and carotenoids ‒ in the leaves of males (70.6 mg%) and female (61.86 mg%) plants in the fruiting phase. Stalks of S. altaiensis contain quite a high amount of tannins (20.1%), pectins (1.49%), protopectins (5.93%) and carotenoids (17.37 mg%). The highest (antioxidant activity) AOA was detected in water (2.03 mg/g) and water-ethanol (1.75 mg/g) extracts from the leaves of male S. altaiensis plants. The AOA of water and water-ethanol extracts from the aerial organs of S. altaiensis is authentically positively connected with the content of all the studied substances, except for pectins.
Впервые изучен состав и содержание флавоноидов и фенолкарбоновых кислот в листьях Sibiraea altaiensis (Laxm.) Schneid., произрастающей в природных условиях и в культуре. Дикорастущие и интродуцированные растения сходны по составу и суммарному содержанию фенольных соединений. Хроматографические профили мужских и женских растений практически одинаковы. В листьях идентифицированы хлорогеновая, кофейная, n-кумариновая, эллаговая, коричная кислоты, кверцетин, гиперозид, изокверцитрин, рутин, авикулярин и астрагалин. Суммарное содержание фенольных соединений в мужских растениях S. altaiensis достигает 21.3 мг/г, в женских ниже - 18.5 мг/г. В листьях мужских растений возрастает содержание флавонолов - кверцетина, гиперозида, изокверцитрина, рутина; в листьях женских растений выше содержание некоторых кислот - хлорогеновой, n-кумаровой, кофейной и эллаговой. В мужских образцах преобладали гликозиды кверцетина (до 12.7 мг/г), что в 1.5 раза выше, чем в женских (8 мг/г). Содержание гликозидов кемпферола и изорамнетина в листьях S. altaiensis из природы сравнительно невысокое. В интродуцированных растениях сумма гликозидов кверцетина снижается в 3 раза (4.1 мг/г), гликозидов изорамнетина - в 2 раза (до 0.2 мг/г), а гликозидов кемпферола, наоборот, повышается до 0.9 мг/г по сравнению с природными растениями.
The expansion of alien plant species in natural communities is now taking a global scale. Since most invasive plants have economically valuable properties, many researchers agree that these plants must be used as new sources of biological resources. The composition and content of biologically active compounds were studied in the leaves of potentially invasive species of Novosibirsk – Physocarpus opulifolius (L.) Maxim. and its ornamental cultivars Ph. opulifolius ‘Diabolo’, Ph. opulifolius ‘Luteus’ by the method of High-Performance Liquid Chromatography. For the first time an analysis of phenolic profiles of introduced, self-sown and cultivated Ph. opulifolius plants has been performed. Presence of 22 phenolic compounds represented by several groups of substances with predominating flavanols (hyperoside, rutin, astragalin and quercetin) has been established. Avicularin has been only revealed in the leaves of cultivars. The sum of identified flavonols greatly varies in different samples, the highest content is in the cultivar Ph. opulifolius ‘Diabolo’ (14.68 mg/g) and self-sown plants of Ph. opulifolius (14.22 mg/g). The content of the sum of identified flavons (vitexin, isovitexin, baicalin) and phenolic acid (сhlorogenic and caffeic acids) is almost the same in all Ph. opulifolius samples under study. The data obtained will allow to widen the idea of the resource potential of the aggressive invasive species and its ornamental cultivars in the secondary areal.
The composition and content of phenolic compounds in the organs of the above-ground part of Agrimonia pilosa Ledeb. depending on the phase of plant development were studied by high performance liquid chromatography (HPLC). Fourteen compounds found in water-ethanol extracts from organs of A. pilosa. Flavonols (kaempferol, astragalin, kaempferol-3-O-β-rutinoside, quercetin, hyperoside and rutin), flavones (apigenin, vitexin, luteolin and luteolin-7-glucoside) and phenolic acids (chlorogenic, caffeic, vanillic and ellagic acids) have been identified. Hyperoside, rutin, and ellagic acid were the major constituents in the extracts from the leaves and the reproductive organs of the species. The highest content of rutin were found in the leaves during vegetative growth and in the inflorescences (2.75 and 2.67 mg/g, respectively). The highest content of hyperoside were observed in leaves in the phase of budding and fruiting (2.02 and 2.25 mg/g). The highest content of ellagic acid were revealed in leaves in the fruiting phase and in buds (3.49 and 2.09 mg/g). The highest total phenolic content was found in the leaves during the fruiting (21.1 mg/g). Unidentified flavonols number 12 and 13 (1.62 and 2.76 mg/g, respectively) were the domi-nant components in the leaves in the growing phase. The total content of phenolic compounds in buds (9.52 mg/g) and in inflorescences (9.80 mg/g) was the same. The total content of phenolic compounds in fruits was two times less (4.24 mg/g) than in buds and inflorescences.
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