A clone encoding mouse glial fibrillary acidic protein (GFAP) was isolated from a cDNA library constructed so as to express the cloned sequences. The library was screened using a GFAP-specific polyclonal antiserum; a single bacterial colony expressing GFAP was identified. The complete sequence of the cDNA insert in this clone is presented, encompassing 2.5 kilobases and specifying >97% of the GFAP amino acid sequence. The clone includes a long (1.4-kilobase) (GFAP). This protein is of particular interest because it represents a specific marker in the development of the central nervous system, its presence distinguishing astrocytes from other glial cell types. Here we describe the isolation and complete sequence of a 2.5-kilobase (kb) cDNA clone encoding mouse GFAP. The structural and evolutionary implications of the predicted amino acid sequence of mouse GFAP are discussed.

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