Acidogenesis of palm oil mill effluent to produce biogas was conducted base on anaerobic digestion process. This process was consisted of four different stages using various types of microorganisms which came from various groups of bacteria. This research studied the effect of hydraulic retention time (HRT) and pH to the change of the organic compounds concentration and solids content of POME to the growth of microorganisms during acidogenesis stage. Initially, the suitable loading up was determined by varying the HRT 6.7; 5.0; and 4.0 days in the continuous stirred tank reactor (CSTR) with mixing rate 50 rpm, pH 6.0 ± 0.2, and room temperature. Next, suitable pH was determined by varying the pH at 5.0; 5.5; and 6.0 with mixing rate 100-110 rpm and temperature 55 o C. Analysis of TS, VS, TSS, VSS, COD, and VFA were conducted in order to study the growth of microorganisms and their abilities in converting organic compound to produce VFA. The highest growth of microorganisms was achieved at HRT 4.0 day with microorganism concentration was 20.62 mg VSS/L and COD reduction was 15.7%. The highest production of total VFA achieved was 5622.72 mg/L at pH 6.0, with the concentration of acetic acid, propionic acid and butyric acid were 2257.34; 975.49; and 2389.90 mg/L, respectively. While degradation VS and COD were 11 and 23%, respectively.
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